Three-dimensional image analysis of the mouse cochlea

被引：7
|
作者：
Wright, Graham D. [1 ]
Horn, Henning F. [1 ]
机构：
[1] ASTAR, Inst Med Biol, 06-06 Immunos, Singapore 138648, Singapore
来源：
DIFFERENTIATION | 2016年 / 91卷 / 4-5期
关键词：
OUTER HAIR-CELLS; INNER-EAR; HEARING-LOSS; CONFOCAL MICROSCOPY; PRESTIN; IMPAIRMENT; DEAFNESS; MODELS;
D O I：
10.1016/j.diff.2016.01.002
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The mouse has proven to be an essential model system for studying hearing loss. A key advantage of the mouse is the ability to image the sensory cells in the cochlea. Many different protocols exist for the dissection and imaging of the cochlea. Here we describe a method that utilizes confocal imaging of whole-mount preparations followed by 3D analysis using the Imaris software. The 3D analysis of confocal stacks has been successfully used for investigating a number of mouse tissues and developmental processes. We propose that this method is also a valuable tool to analyze the cellular and tissue organization of the sensory hair cells in the cochlea. (C) 2016 International Society of Differentiation. Published by Elsevier B.V. All rights reserved.
引用
收藏
页码：104 / 108
页数：5
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