Anticancer potential of steviol in MCF-7 human breast cancer cells

被引：32
|
作者：
Gupta, Ena [1 ]
Kaushik, Shweta [2 ]
Purwar, Shalini [1 ]
Sharma, Ramesh [2 ]
Balapure, Anil K. [2 ]
Sundaram, Shanthy [1 ]
机构：
[1] Univ Allahabad, Ctr Biotechnol, Allahabad, Uttar Pradesh, India
[2] Cent Drug Res Inst, Div Biochem, Lucknow, Uttar Pradesh, India
来源：
PHARMACOGNOSY MAGAZINE | 2017年 / 13卷 / 51期
关键词：
Apoptosis; Michigan Cancer Foundation-7; Stevia rebaudiana; Steviol; APOPTOSIS; ACTIVATION;
D O I：
10.4103/pm.pm_29_17
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Objective: This study aimed to investigate the cytotoxicity, apoptosis induction, and mechanism of action of steviol on human breast cancer cells (Michigan Cancer Foundation-7 [MCF-7]). Materials and Methods: Sulforhodamine-B assay was performed to analyze cytotoxic potential of Steviol whereas flow cytometer was used to analyze cell cycle, apoptosis, and reactive oxygen species generation. Results: Studying the viability of cells confirms the IC(50)of Steviol in MCF-7 cells which was 185 mu M. The data obtained from fluorescence-activated cell sorter analysis reveal Steviol-mediated G2/M-phase arrest (P < 0.05) in addition to the presence of evident sub-G0/G1 peak (P < 0.05) in the MCF-7 cells, signifying the ongoing apoptosis. Conclusion: Thus, results suggest that induction of apoptosis in MCF-7 cells was due to dose-dependent effect of Steviol. Our first in vitro findings indicate Steviol as a promising candidate for the treatment of breast cancer.
引用
收藏
页码：345 / 350
页数：6
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