Molecular characterization of enrofloxacin resistant Actinobacillus pleuropneumoniae isolates

被引：36

作者：

Wang, Yu-Chih ^{[1
]}

Chan, Jacky Peng-Wen ^{[1
]}

Yeh, Kuang-Sheng ^{[3
]}

Chang, Chao-Chin ^{[2
]}

Hsuan, Shih-Ling ^{[4
]}

Hsieh, Yi-Mei ^{[2
]}

Chang, Yi-Chih ^{[1
,5
]}

Lai, Tan-Chen ^{[1
,6
]}

Lin, Wen-Hsin ^{[7
]}

Chen, Ter-Hsin ^{[2
,4
]}

机构：

[1] Natl Chung Hsing Univ, Dept Vet Med, Taichung 40227, Taiwan

[2] Natl Chung Hsing Univ, Coll Vet Med, Grad Inst Vet Publ Hlth, Taichung 40227, Taiwan

[3] Taipei Med Univ, Coll Med, Sch Med, Dept Microbiol & Immunol, Taipei, Taiwan

[4] Natl Chung Hsing Univ, Coll Vet Med, Grad Inst Vet Pathobiol, Taichung 40227, Taiwan

[5] China Med Univ, Dept Med Lab Sci & Biotechnol, Taichung, Taiwan

[6] Cent Taiwan Univ Sci & Technol, Dept Med Lab Sci & Biotechnol, Taichung, Taiwan

[7] China Med Univ, Sch Pharm, Taichung, Taiwan

来源：

VETERINARY MICROBIOLOGY | 2010年 / 142卷 / 3-4期

关键词：

Enrofloxacin resistance; Actinobacillus pleuropneumoniae; Quinolone resistance-determining region; Efflux pump; MEDIATED QUINOLONE RESISTANCE; ANTIMICROBIAL SUSCEPTIBILITY; ESCHERICHIA-COLI; PARC MUTATIONS; EFFLUX PUMP; SALMONELLA; IDENTIFICATION; PREVALENCE; SEROTYPE-2; STRAINS;

D O I：

10.1016/j.vetmic.2009.09.067

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Enrofloxacin (ER) resistant Actinobacillus pleuropneumoniae strains emerged in Taiwan in 2002. The mechanism of ER resistance in A. pleuropneumoniae has not yet been reported. A total of 48 A. pleuropneumoniae isolates were obtained from the lungs of pigs with pleuropneumonia in Taiwan between September 2007 and April 2008. Twenty-nine isolates were found to be resistant to enrofloxacin. To understand the mechanisms of A. pleuropneumoniae's resistance to ER, enrofloxacin susceptibility of the isolates along with the mutations of the quinolone resistance-determining region (QRDR) of DNA gyrase and topoisomerase IV, qnr genes were analyzed. Enrofloxacin resistant isolates were found to carry at least one mutation in the QRDR of gyrA, leading to amino acid changes at codon 83 or 87. Efflux pump inhibitor (Phe-Arg-beta-naphthylamide) decreased enrofloxacin minimum inhibitory concentration 2-16-fold, suggesting participation of efflux in ER resistance. Plasmid mediated quinolone resistance genes qnr were not detected in these isolates. In conclusion, enrofloxacin resistance of A. pleuropneumoniae may be linked to multiple target gene mutations and active effluxs. (C) 2009 Elsevier B.V. All rights reserved.

引用

页码：309 / 312

页数：4

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