Transcriptome Analysis Reveals Potential Antioxidant Defense Mechanisms in Antheraea pernyi in Response to Zinc Stress

被引：41

作者：

Liu, Yu ^{[1
,2
,4
]}

Xin, Zhao-Zhe ^{[1
,4
]}

Song, Jiao ^{[3
]}

Zhu, Xiao-Yu ^{[1
]}

Liu, Qiu-Ning ^{[1
]}

Zhang, Dai-Zhen ^{[1
]}

Tang, Bo-Ping ^{[1
]}

Zhou, Chun-Lin ^{[1
]}

Dai, Li-Shang ^{[2
]}

机构：

[1] Yancheng Teachers Univ, Jiangsu Synthet Innovat Ctr Coastal Bioagr, Jiangsu Key Lab Bioresources Saline Soils, Sch Ocean & Biol Engn,Jiangsu Prov Key Lab Coasta, Yancheng 224051, Peoples R China

[2] Wenzhou Med Univ, Sch Pharmaceut Sci, Wenzhou 325035, Peoples R China

[3] Anhui Agr Univ, Coll Life Sci, Hefei 230036, Anhui, Peoples R China

[4] Nanjing Univ Technol, Coll Biotechnol & Pharmaceut Engn, Nanjing 210009, Jiangsu, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2018年 / 66卷 / 30期

基金：

中国国家自然科学基金;

关键词：

Antheraea pernyi; antioxidant mechanisms; heavy metals; zinc; transcriptome; CHINESE OAK SILKWORM; OXIDATIVE STRESS; PEROXISOMES; METABOLISM; DIAPAUSE; IDENTIFICATION; TRANSMISSION; ANNOTATION; SIGNALS; PROTEIN;

D O I：

10.1021/acs.jafc.8b01645

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The growth and development of the Chinese oak silkworm, Antheraea pernyi, are strongly influenced by environmental conditions, including heavy metal pollution. An excess of heavy metals causes cellular damage through the production of free radical reactive oxygen species. In this study, transcriptome analysis was performed to investigate global gene expression when A. pernyi was exposed to zinc infection. With RNA sequencing (RNA-Seq), a total of 25 795 510 and 38 158 855 clean reads were obtained from zinc-treated and control fat body libraries, respectively. We identified 2399 differential expression genes (DEGs) (1845 upregulated and 544 downregulated genes) in the zinc-treated library. In addition, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that these DEGs were related to the peroxisome pathway that was associated with antioxidant defense. Our results suggest that fat bodies of A. pernyi constitute a strong antioxidant defense against heavy metal contamination.

引用

页码：8132 / 8141

页数：10

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