Injection of day 2 embryo culture supernatant into the uterine cavity did not improve the pregnancy rate of day 3 embryo transfer in patients who underwent in vitro fertilization-embryo transfer: a randomized clinical trial

Objective: To evaluate the effectiveness of uterine cavity injection of day 2 embryo culture supernatant before day 3 embryo transfer in patients who are undergoing in vitro fertilization-embryo transfer. Design: Randomized controlled trial. Setting: A reproductive medical centre. Patient(s): A total of 90 infertile women, 45 of them in the study group and 45 in the control group. Intervention(s): Uterine cavity infection of day 2 embryo culture supernatant before day 3 embryo transfer. Main Outcome Measure(s): Embryo implantation rate and pregnancy rate. Result(s): The pregnancy versus implantation rates in the study group and control group were 48.9% versus 44.4% and 27.3% versus 22.1%, respectively. Although both pregnancy and implantation rates in the study group were higher than those in the control group, no statistically significant differences were found in these two parameters. Conclusion(s): Injection of day 2 embryo culture supernatant into the uterine cavity cannot improve the implantation and pregnancy rates of day 3 embryo transfer. (Fertil Steril (R) 2010;93:2216-21. (C)2010 by American Society for Reproductive Medicine.)