The effects and mechanisms of GM-CSF on endometrial regeneration

被引：16

作者：

Liu, Juan ^{[1
,2
]}

Ying, Yanyun ^{[1
,2
]}

Wang, Siwen ^{[1
,2
]}

Li, Jingyi ^{[1
,2
]}

Xu, Jinqun ^{[1
,2
]}

Lv, Pingping ^{[1
,2
]}

Chen, Jianhua ^{[3
]}

Zhou, Caiyun ^{[3
]}

Liu, Yifeng ^{[1
,2
]}

Wu, Yiqing ^{[1
,2
]}

Huang, Yun ^{[1
,2
]}

Chen, Yao ^{[1
,2
]}

Chen, Lifen ^{[1
,2
,4
]}

Tu, Shijiong ^{[1
,2
,5
]}

Zhao, Wei ^{[1
,2
]}

Yang, Min ^{[1
,2
]}

Hu, Yanjun ^{[1
,2
]}

Zhang, Runju ^{[1
,2
]}

Zhang, Dan ^{[1
,2
]}

机构：

[1] Zhejiang Univ, Minist Educ, Sch Med, Key Lab Reprod Genet, Hangzhou 310006, Zhejiang, Peoples R China

[2] Zhejiang Univ, Womens Hosp, Sch Med, Dept Reprod Endocrinol, Hangzhou 310006, Zhejiang, Peoples R China

[3] Zhejiang Univ, Womens Hosp, Sch Med, Dept Pathol, Hangzhou 310006, Zhejiang, Peoples R China

[4] Huzhou Matern & Child Care Hosp, Huzhou, Peoples R China

[5] Ningbo Women & Childrens Hosp, Ningbo, Zhejiang, Peoples R China

来源：

CYTOKINE | 2020年 / 125卷

基金：

中国国家自然科学基金;

关键词：

GM-CSF; Regeneration; Thin endometrium; Endometrial glandular and stromal cells; PI3K/Akt; COLONY-STIMULATING FACTOR; IN-VITRO FERTILIZATION; THIN ENDOMETRIUM; ALPHA-RECEPTOR; STROMAL CELLS; BETA-RECEPTOR; PREGNANCY; THICKNESS; STEM; EXPRESSION;

D O I：

10.1016/j.cyto.2019.154850

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: Endometrial injury can result in thin endometrium and subfertility. Granulocyte macrophage colony stimulating factor (GM-CSF) contributes to tissue repair, but its role in endometrial regeneration has not been investigated. Methods: To determine the effect of GM-CSF on endometrial regeneration, we established a mouse model of thin endometrium by uterine perfusion with 20 mu L 90% ethanol. Thin endometrium in mice was featured by lowered endometrial thickness, decreased expression of Ki67 in glandular cells, and a reduced number of implantation sites. To explore the mechanism of GM-CSF on endometrial regeneration, endometrium was obtained from patients undergoing hysterectomy or hysteroscopy and endometrial biopsy. Effects of GM-CSF on primary cultured human endometrial glandular and stromal cells were examined by the 5-bromo-2'-deoxyuridine (BrdU) proliferation assay and transwell migration assay, followed by exploration of the potential signaling pathway. Results: GM-CSF intraperitoneal (i.p.) injection significantly increased endometrial thickness, expression of Ki67 in endometrial glandular cells, and the number of implantation sites. GM-CSF significantly promoted proliferation of primary human endometrial glandular cells and migration of stromal cells. GM-CSF activated p-Akt and increased expressions of p70S6K and c-Jun, which were blocked by LY294002. Conclusion: We found that GM-CSF could improve endometrial regeneration, possibly through activating PI3K/Akt signaling pathway.

引用

页数：10

共 43 条

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