Alignment of conduits for the nascent polypeptide chain in the Ribosome-Sec61 complex

被引：288

作者：

Beckmann, R

Bubeck, D

Grassucci, R

Penczek, P

Verschoor, A

Blobel, G

Frank, J

机构：

[1] NEW YORK STATE DEPT HLTH,WADSWORTH CTR,ALBANY,NY 12201

[2] SUNY ALBANY,DEPT BIOMED SCI,ALBANY,NY 12201

来源：

SCIENCE | 1997年 / 278卷 / 5346期

关键词：

D O I：

10.1126/science.278.5346.2123

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

An oligomer of the Sec61 trimeric complex is thought to form the protein-conducting channel for protein transport across the endoplasmic reticulum. A purified yeast Sec61 complex bound to monomeric yeast ribosomes as an oligomer in a saturable fashion. Cryo-electron microscopy of the ribosome-Sec61 complex and a three-dimensional reconstruction showed that the Sec61 oligomer is attached to the large ribosomal subunit by a single connection. Moreover, a funnel-shaped pore in the Sec61 oligomer aligned with the exit of a tunnel traversing the large ribosomal subunit, strongly suggesting that both structures function together in the translocation of proteins across the endoplasmic reticulum membrane.

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页码：2123 / 2126

页数：4

相关论文

共 28 条

[1] 2 NOVEL RELATED YEAST NUCLEOPORINS NUP170P AND NUP157P - COMPLEMENTATION WITH THE VERTEBRATE HOMOLOG NUP155P AND FUNCTIONAL INTERACTIONS WITH THE YEAST NUCLEAR PORE-MEMBRANE PROTEIN POM152P [J].

AITCHISON, JD ;

ROUT, MP ;

MARELLI, M ;

BLOBEL, G ;

WOZNIAK, RW .

JOURNAL OF CELL BIOLOGY, 1995, 131 (05) :1133-1148

[2] NASCENT POLYPEPTIDE-CHAINS EXIT THE RIBOSOME IN THE SAME RELATIVE POSITION IN BOTH EUKARYOTES AND PROCARYOTES [J].

BERNABEU, C ;

TOBIN, EM ;

FOWLER, A ;

ZABIN, I ;

LAKE, JA .

JOURNAL OF CELL BIOLOGY, 1983, 96 (05) :1471-1474

[3] TRANSFER OF PROTEINS ACROSS MEMBRANES .1. PRESENCE OF PROTEOLYTICALLY PROCESSED AND UNPROCESSED NASCENT IMMUNOGLOBULIN LIGHT-CHAINS ON MEMBRANE-BOUND RIBOSOMES OF MURINE MYELOMA [J].

BLOBEL, G ;

DOBBERSTEIN, B .

JOURNAL OF CELL BIOLOGY, 1975, 67 (03) :835-851

[4] Determination of the fold of the core protein of hepatitis B virus ky electron cryomicroscopy [J].

Bottcher, B ;

Wynne, SA ;

Crowther, RA .

NATURE, 1997, 386 (6620) :88-91

[5] THE SIGNAL SEQUENCE MOVES THROUGH A RIBOSOMAL TUNNEL INTO A NONCYTOPLASMIC AQUEOUS ENVIRONMENT AT THE ER MEMBRANE EARLY IN TRANSLOCATION [J].

CROWLEY, KS ;

REINHART, GD ;

JOHNSON, AE .

CELL, 1993, 73 (06) :1101-1115

[6] SECRETORY PROTEINS MOVE THROUGH THE ENDOPLASMIC-RETICULUM MEMBRANE VIA AN AQUEOUS, GATED PORE [J].

CROWLEY, KS ;

LIAO, SR ;

WORRELL, VE ;

REINHART, GD ;

JOHNSON, AE .

CELL, 1994, 78 (03) :461-471

[7] A YEAST MUTANT DEFECTIVE AT AN EARLY STAGE IN IMPORT OF SECRETORY PROTEIN PRECURSORS INTO THE ENDOPLASMIC-RETICULUM [J].

DESHAIES, RJ ;

SCHEKMAN, R .

JOURNAL OF CELL BIOLOGY, 1987, 105 (02) :633-645

[8] CRYO-ELECTRON MICROSCOPY OF VITRIFIED SPECIMENS [J].

DUBOCHET, J ;

ADRIAN, M ;

CHANG, JJ ;

HOMO, JC ;

LEPAULT, J ;

MCDOWALL, AW ;

SCHULTZ, P .

QUARTERLY REVIEWS OF BIOPHYSICS, 1988, 21 (02) :129-228

[9] A MODEL OF PROTEIN-SYNTHESIS BASED ON CRYOELECTRON MICROSCOPY OF THE E-COLI RIBOSOME [J].

FRANK, J ;

ZHU, J ;

PENCZEK, P ;

LI, YH ;

SRIVASTAVA, S ;

VERSCHOOR, A ;

RADERMACHER, M ;

GRASSUCCI, R ;

LATA, RK ;

AGRAWAL, RK .

NATURE, 1995, 376 (6539) :441-444

[10] TRANSLOCATION OF SECRETORY PROTEINS ACROSS THE MICROSOMAL MEMBRANE OCCURS THROUGH AN ENVIRONMENT ACCESSIBLE TO AQUEOUS PERTURBANTS [J].

GILMORE, R ;

BLOBEL, G .

CELL, 1985, 42 (02) :497-505