Multilevel profiling and identification of Dalbergia odorifera and Dalbergia stevensonii by FTIR, NMR and GC/MS

As a rare and valuable wood and herbal material, Dalbergia odorifera is often counterfeited by Dalbergia stevensonii in the market. For the confident chemical identification of D. odorifera and D. stevensonii, the ethanol-benzene extractives are characterized by multiple metabolomics tools, including Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (NMR), and gas chromatography mass spectrometry (GC/MS). Conventional FT1R spectroscopy, second derivative infrared (SD-IR) spectroscopy and two-dimensional correlation infrared (2D-IR) spectroscopy are combined to interpret the functional groups of the ethanol-benzene extractives. Fingerprint-like characteristics make FTIR a rapid and accurate method to distinguish D. odorifera from D. stevensonii. Chemical differences of the extractives revealed by FT1R methods can be further confirmed by H-1 NMR and C-13 NMR. Meanwhile, the volatile compounds in the extractives can be identified by GC/MS. The combination of FT1R, NMR and GC/MS makes it possible to obtain the multiple profiles of the ethanol-benzene extractives, which is essential for the confident chemical identification of D. odorifera and D. stevensonii. (C) 2018 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.