Bovine viral diarrhoea virus: its effects on ovarian function in the cow

被引:36
作者
Fray, MD [1 ]
Mann, GE
Clarke, MC
Charleston, B
机构
[1] Inst Anim Hlth, Embryol Grp, Div Environm Microbiol, Newbury RG20 7NN, Berks, England
[2] Univ Nottingham, Sutton Bonington LE12 5RD, Leics, England
关键词
bovine viral diarrhoea virus; cow; steroids; oocytes; ovary;
D O I
10.1016/S0378-1135(00)00275-3
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Bovine viral diarrhoea virus (BVDV) is a major cattle pathogen responsible for a spectrum of symptoms, including reproductive failure. In this paper we investigate how BVDV interacts with the ovary. The viruses' tropism for the pre-ovulatory oocyte was studied by indirect immunohistochemistry. Two monoclonal antibodies, raised against the non-structural protein NS3 and the envelope glycoprotein E2 were used to probe cryo-sections cue from the ovaries of three persistently infected heifers. NS3 and E2 antigens were widely distributed within the ovarian stroma and follicular cells. NS3 was also localised within the proportion of oocytes. Overall 18.7% of the oocyte population had detectable levels of NS3. What is more, the proportion of antigen positive oocytes remained constant (P > 0.05) throughout the different stages of oocyte maturation. In a subsequent study seven cows were challenged with non-cytopathogenic BVDV (strain Pe515: 5 x 10(6) TCID50) to determine the oestradiol and progesterone responses to an acute infection. The sensitivity of the endogenous luteolytic mechanism was also established by analysing plasma prostaglandin F2 alpha metabolite (PGFM) levels following an exogenous oxytocin (50 TU) challenge. The inoculation was given 2 days before a synchronised oestrus and was timed to ensure that viraemia occurred during the initial stage of corpora luteal development. Seven cows inoculated with non-infectious culture medium served as control animals and remained BVDV naive throughout the study. The BVDV challenge was followed by leucopenia, viraemia and seroconversion. The virus also significantly (P < 0.01) reduced plasma oestradiol levels between day 6 and day ii post-inoculation (i.e. between day 4 and day 9 post-oestrus). However, the infection did not alter (P > 0.05) progesterone secretion throughout the oestrous cycle or the plasma concentration of PGFM. These data indicate that bovine follicular cells and oocytes are permissive to BVDV at all stages of follicular development. They also show that a transient fall in oestradiol secretion may accompany an acute infection. In conclusion, this work has identified two potential routes through which BVDV can reduce fertility in the cow, namely impairment of oocyte quality and disruption of gonadal steroidogenesis. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:185 / 194
页数:10
相关论文
共 34 条
  • [1] THE POTENTIAL RELEVANCE OF CYTOKINES TO OVARIAN PHYSIOLOGY - THE EMERGING ROLE OF RESIDENT OVARIAN-CELLS OF THE WHITE BLOOD-CELL SERIES
    ADASHI, EY
    [J]. ENDOCRINE REVIEWS, 1990, 11 (03) : 454 - 464
  • [2] ANDERSON CA, 1987, LAB INVEST, V57, P168
  • [3] CALVES BORN AFTER EMBRYO TRANSFER FROM DONORS PERSISTENTLY INFECTED WITH BVD VIRUS
    BAK, A
    CALLESEN, H
    MEYLING, A
    GREVE, T
    [J]. VETERINARY RECORD, 1992, 131 (02) : 37 - 37
  • [4] DIAGNOSIS OF NATURALLY-OCCURRING BOVINE VIRAL DIARRHEA VIRUS-INFECTIONS IN RUMINANTS USING MONOCLONAL ANTIBODY-BASED IMMUNOHISTOCHEMISTRY
    BASZLER, TV
    EVERMANN, JF
    KAYLOR, PS
    BYINGTON, TC
    DILBECK, PM
    [J]. VETERINARY PATHOLOGY, 1995, 32 (06) : 609 - 618
  • [5] BOOTH PJ, 1995, J REPROD FERTIL, V105, P17, DOI 10.1530/jrf.0.1050017
  • [6] Embryo transfer from donor cattle persistently infected with bovine viral diarrhea virus
    Brock, KV
    Lapin, DR
    Skrade, DR
    [J]. THERIOGENOLOGY, 1997, 47 (04) : 837 - 844
  • [7] Expression of non-cytopathogenic bovine viral diarrhoea virus (BVDV) in oocytes and follicles of persistently infected cattle
    Brownlie, J
    Booth, PJ
    Stevens, DA
    Collins, ME
    [J]. VETERINARY RECORD, 1997, 141 (13) : 335 - 337
  • [8] BOVINE VIRUS DIARRHEA-MUCOSAL DISEASE VIRUS - PATHOGENICITY FOR THE FETAL CALF FOLLOWING MATERNAL INFECTION
    DONE, JT
    TERLECKI, S
    RICHARDSON, C
    HARKNESS, JW
    SANDS, JJ
    PATTERSON, DSP
    SWEASEY, D
    SHAW, IG
    WINKLER, CE
    DUFFELL, SJ
    [J]. VETERINARY RECORD, 1980, 106 (23) : 473 - 479
  • [9] IMPACT OF BOVINE VIRAL DIARRHEA VIRUS ON REPRODUCTIVE-PERFORMANCE IN CATTLE
    DUBOVI, EJ
    [J]. VETERINARY CLINICS OF NORTH AMERICA-FOOD ANIMAL PRACTICE, 1994, 10 (03) : 503 - 514
  • [10] Immunohistochemical evidence for the localization of bovine viral diarrhea virus, a single-stranded RNA virus, in ovarian oocytes in the cow
    Fray, MD
    Prentice, H
    Clarke, MC
    Charleston, B
    [J]. VETERINARY PATHOLOGY, 1998, 35 (04) : 253 - 259