Antibacterial activity and mechanism of chitosan with ultra high molecular weight

被引：188

作者：

Li, Jianhui ^{[1
,2
]}

Wu, Yiguang ^{[1
]}

Zhao, Liqing ^{[1
]}

机构：

[1] Shenzhen Univ, Coll Chem & Environm Engn, Shenzhen 518060, Guangdong, Peoples R China

[2] Xi An Jiao Tong Univ, Sch Sci, Dept Chem, Xian 710049, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2016年 / 148卷

关键词：

Chitosan; Ultra high molecular weight; Bacteriostatic and bactericidal activity; Antibacterial mechanism; AID;

D O I：

10.1016/j.carbpol.2016.04.025

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Chitosan with different degree of deacetylation (DD) and ultra high molecular weight (MW >10(6)) was prepared from beta-chitin by mild deacetylation. The effects of DD of chitosan and pH value of its solution/suspension on its antibacterial activity were investigated. The results showed that the optimal pH value was 6.0 for the highest bactericidal activity. The antibacterial activity against Escherichia coli and Staphylococcus aureus of chitosan solution at pH 6.0 enhanced as the DD of chitosan increased. Same as chitosan with low MW, the antibacterial activity of chitosan with high MW in acidic solution was also due to the amino protonation and subsequently cationic formation. Its ultra long molecular chain was propitious to coat and bind the E. coli and S. aureus, and highly enhanced its antibacterial activity. E. coli and S. aureus were at first restrained and then killed by chitosan and the cells were ruptured and decomposed gradually. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：200 / 205

页数：6

共 12 条

[1] Antibacterial property and characterization of cotton fabric treated with chitosan/AgCl-TiO2 colloid [J].

Arain, Rabia Almas ;

Khatri, Zeeshan ;

Memon, Muhammad Hanif ;

Kim, Ick-Soo .

CARBOHYDRATE POLYMERS, 2013, 96 (01) :326-331

[2] Preparation and Biomedical Applications of Chitin and Chitosan Nanofibers [J].

Azuma, Kazuo ;

Ifuku, Shinsuke ;

Osaki, Tomohiro ;

Okamoto, Yoshiharu ;

Minami, Saburo .

JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, 2014, 10 (10) :2891-2920

[3] pH Effects on solubility, zeta potential, and correlation between antibacterial activity and molecular weight of chitosan [J].

Chang, Shun-Hsien ;

Lin, Hong-Ting Victor ;

Wu, Guan-James ;

Tsai, Guo Jane .

CARBOHYDRATE POLYMERS, 2015, 134 :74-81

[4] Chitosan coated cotton gauze for antibacterial water filtration [J].

Ferrero, Franco ;

Periolatto, Monica ;

Vineis, Claudia ;

Varesano, Alessio .

CARBOHYDRATE POLYMERS, 2014, 103 :207-212

[5] Antimicrobial effect of chitooligosaccharides produced by bioreactor [J].

Jeon, YJ ;

Park, PJ ;

Kim, SK .

CARBOHYDRATE POLYMERS, 2001, 44 (01) :71-76

[6] Low molecular weight chitosans -: Preparation with the aid of pronase, characterization and their bactericidal activity towards Bacillus cereus and Escherichia coli [J].

Kumar, Acharya B. Vishu ;

Varadaraj, Mandyam C. ;

Gowda, Lalitha R. ;

Tharanathan, Rudrapatnam N. .

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2007, 1770 (04) :495-505

[7] Low molecular weight chitosan prepared with the aid of cellulase, lysozyme and chitinase: Characterisation and antibacterial activity [J].

Lin, Shih-Bin ;

Lin, Yi-Chun ;

Chen, Hui-Huang .

FOOD CHEMISTRY, 2009, 116 (01) :47-53

[8] Effect of MW and concentration of chitosan on antibacterial activity of Escherichia coli [J].

Liu, Nan ;

Chen, Xi-Guang ;

Park, Hyun-Jin ;

Liu, Chen-Guang ;

Liu, Cheng-Sheng ;

Meng, Xiang-Hong ;

Yu, Le-Jun .

CARBOHYDRATE POLYMERS, 2006, 64 (01) :60-65

[9] Preparation and antibacterial activity of chitosan nanoparticles [J].

Qi, LF ;

Xu, ZR ;

Jiang, X ;

Hu, CH ;

Zou, XF .

CARBOHYDRATE RESEARCH, 2004, 339 (16) :2693-2700

[10]

Rajendran K., 2013, IND CROP PROD, V47, P8

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