Top-Down Mass Spectrometry for Sequencing of Larger (up to 61 nt) RNA by CAD and EDD

被引：63

作者：

Taucher, Monika

Breuker, Kathrin ^{[1
]}

机构：

[1] Univ Innsbruck, Inst Organ Chem, A-6020 Innsbruck, Austria

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 2010年 / 21卷 / 06期

基金：

奥地利科学基金会;

关键词：

ELECTRON DETACHMENT DISSOCIATION; INFRARED MULTIPHOTON DISSOCIATION; COLLISION-INDUCED DISSOCIATION; MULTIPLY DEPROTONATED RNA; CAPTURE DISSOCIATION; GLYCOSAMINOGLYCAN TETRASACCHARIDES; PROTEIN CHARACTERIZATION; NUCLEIC-ACIDS; CHARGE-STATE; IONS;

D O I：

10.1016/j.jasms.2010.02.025

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have studied the effect of solution additives on hydrolysis and charge state distribution in ESI MS of RNA. Lower and higher charge state ions can be electrosprayed from solutions containing 25 mM piperidine/25 mM imidazole and 1% vol. triethylamine, respectively, with base-catalyzed hydrolysis rates that are sufficiently slow to perform MS/MS experiments. These lower and higher charge state ions are suitable as precursors for CAD and EDD, respectively. We demonstrate nearly complete sequence coverage for 61 nt RNA dissociated by CAD, and 34 nt RNA dissociated by EDD, and suggest a mechanism for backbone fragmentation in EDD of RNA. (J Am Soc Mass Spectrom 2010, 21, 918-929) (C) 2010 American Society for Mass Spectrometry

引用

收藏

页码：918 / 929

页数：12

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