Poly(2-Hydroxyethyl Methacrylate)-b-Poly (L-Lysine) Cationic Hybrid Materials for Non-Viral Gene Delivery in NIH 3T3 Mouse Embryonic Fibroblasts

被引：13

作者：

Johnson, Renjith P. ^{[1
]}

Uthaman, Saji ^{[2
]}

John, Johnson V. ^{[1
]}

Heo, Min Seon ^{[1
]}

Park, In Kyu ^{[2
]}

Suh, Hongsuk ^{[3
]}

Kim, Il ^{[1
]}

机构：

[1] Pusan Natl Univ, Dept Polymer Sci & Engn, PLUS Ctr Adv Chem Technol BK21, Pusan 609735, South Korea

[2] Chonnam Natl Univ, Dept Biomed Sci, PLUS Ctr Creat Biomed Scientists BK21, Sch Med, Kwangju 501746, South Korea

[3] Pusan Natl Univ, Chem Inst Funct Mat, Dept Chem, Pusan 609735, South Korea

来源：

MACROMOLECULAR BIOSCIENCE | 2014年 / 14卷 / 09期

基金：

新加坡国家研究基金会;

关键词：

ATRP; cationic block copolymers; gene delivery; polypeptide; polyplex; POLY-L-LYSINE; STEM-CELLS; POLYMERIZATION; COPOLYMERS; SAFE;

D O I：

10.1002/mabi.201400071

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to develop efficient and nontoxic gene delivery vectors, a series of biocompatible block copolymers, poly[(2-hydroxyethyl methacrylate)(40)-block-(L-lysine)(n)] (n = 40, 80, 120, 150), are prepared by combining an atom transfer radical polymerization of 2-hydroxyethyl methacrylate with a ring-opening polymerization of N-epsilon-(carbobenzoxy)-L-lysine N-carboxyanhydride. The block copolymers are successfully condensed with plasmid DNA (pDNA) into nanosized (<200 nm) polyplexes. As a representative sample, p-(HEMA)(40)-b-p(lys)(150) is utilized to confirm the effective cellular and nuclear uptake of pDNA. The polymer/pDNA polyplexes exhibit very low cytotoxicity and enhanced transfection activity by being easily taken up into mouse embryonic fibroblast cell line (NIH 3T3). Thus, the chimeric block copolymers provide a means for developing versatile nonviral gene vectors harboring the ideal requirements of low cytotoxicity, good stability, and high transfection efficiency for gene therapy.

引用

页码：1239 / 1248

页数：10

共 33 条

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