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Immobilization of glucose oxidase onto Langmuir-Blodgett films of poly-3-hexylthiophene
被引:32
作者:
Singhal, R
Chaubey, A
Srikhirin, T
Aphiwantrakul, S
Pandey, SS
Malhotra, BD
机构:
[1] Natl Phys Lab, Biomol Elect & Conducting Polymer Res Grp, New Delhi 110012, India
[2] Mahidol Univ, Dept Phys, Nanosci & Nanotechnol Capabil Bldg Unit, Bangkok 10700, Thailand
[3] Kyushu Inst Technol, Dept Comp Sci, Iizuka, Fukuoka 820, Japan
关键词:
Langmuir-Blodgett films;
P3HT;
glucose sensor;
AFM;
X-ray diffraction;
D O I:
10.1016/S1567-1739(02)00215-8
中图分类号:
T [工业技术];
学科分类号:
08 ;
摘要:
Monolayers of poly(3-hexyl thiophene) (P3HT) were obtained by dispensing a solution of P3HT with stearic acid (SA) in chloroform at air-water interface using Joyce-Loebl LB trough. These films were transferred onto indium-tin-oxide coated glass plate by vertical dipping method. Enzyme glucose oxidase (GOX) was immobilized on the films using LB technique. These films were characterized by X-ray diffraction and atomic force microscopic techniques. Photometric response of these P3HT/SA/GOX films was obtained as a function of glucose concentration. These P3HT/SA/GOX LB films show a linearity from 100 to 500 mg/dl of glucose concentration. (C) 2002 Elsevier Science B.V. All rights reserved.
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页码:275 / 279
页数:5
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