Immobilization of glucose oxidase onto Langmuir-Blodgett films of poly-3-hexylthiophene

被引：32
作者：
Singhal, R
Chaubey, A
Srikhirin, T
Aphiwantrakul, S
Pandey, SS
Malhotra, BD
机构：
[1] Natl Phys Lab, Biomol Elect & Conducting Polymer Res Grp, New Delhi 110012, India
[2] Mahidol Univ, Dept Phys, Nanosci & Nanotechnol Capabil Bldg Unit, Bangkok 10700, Thailand
[3] Kyushu Inst Technol, Dept Comp Sci, Iizuka, Fukuoka 820, Japan
来源：
CURRENT APPLIED PHYSICS | 2003年 / 3卷 / 2-3期
关键词：
Langmuir-Blodgett films; P3HT; glucose sensor; AFM; X-ray diffraction;
D O I：
10.1016/S1567-1739(02)00215-8
中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
Monolayers of poly(3-hexyl thiophene) (P3HT) were obtained by dispensing a solution of P3HT with stearic acid (SA) in chloroform at air-water interface using Joyce-Loebl LB trough. These films were transferred onto indium-tin-oxide coated glass plate by vertical dipping method. Enzyme glucose oxidase (GOX) was immobilized on the films using LB technique. These films were characterized by X-ray diffraction and atomic force microscopic techniques. Photometric response of these P3HT/SA/GOX films was obtained as a function of glucose concentration. These P3HT/SA/GOX LB films show a linearity from 100 to 500 mg/dl of glucose concentration. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：275 / 279
页数：5
相关论文
共 21 条
[1]   An optical gas sensor based on polyaniline Langmuir-Blodgett films [J].
Agbor, NE ;
Cresswell, JP ;
Petty, MC ;
Monkman, AP .
SENSORS AND ACTUATORS B-CHEMICAL, 1997, 41 (1-3) :137-141
[2]   Polythiophene Langmuir-Blodgett films: How do they order? [J].
Bardosova, M ;
Stiller, B ;
Tredgold, RH ;
Woolley, M ;
Hodge, P ;
Brehmer, L .
THIN SOLID FILMS, 1996, 284 :450-452
[3]   ELECTROCHEMICAL RESPONSE OF AN ENZYME-LOADED POLYANILINE FILM [J].
COOPER, JC ;
HALL, EAH .
BIOSENSORS & BIOELECTRONICS, 1992, 7 (07) :473-485
[4]   A study of Langmuir and Langmuir-Blodgett films of polyaniline [J].
Dhanabalan, A ;
Dabke, RB ;
Kumar, NP ;
Talwar, SS ;
Major, S ;
Lal, R ;
Contractor, AQ .
LANGMUIR, 1997, 13 (16) :4395-4400
[5]   Preparation of conducting layers of substituted polyacetylenes by solid-phase polymerization of Langmuir-Blodgett layers [J].
Dultsev, FN ;
Repinsky, SM ;
Sveshnikova, LL .
THIN SOLID FILMS, 2000, 359 (02) :239-243
[6]  
DUROVSKY TB, 1993, BIOSENS BIOELECTRON, V8, P377
[7]   CHARGE PROPAGATION THROUGH A REDOX POLYMER FILM CONTAINING ENZYMES - EFFECTS OF ENZYME LOADING, PH AND SUPPORTING ELECTROLYTE [J].
ELMGREN, M ;
LINDQUIST, SE ;
SHARP, M .
JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1993, 362 (1-2) :227-235
[8]   IMMOBILIZATION OF GLUCOSE-OXIDASE IN FERROCENE-MODIFIED PYRROLE POLYMERS [J].
FOULDS, NC ;
LOWE, CR .
ANALYTICAL CHEMISTRY, 1988, 60 (22) :2473-2478
[9]   Coimmobilization of urease and glutamate dehydrogenase in electrochemically prepared polypyrrole-polyvinyl sulfonate films [J].
Gambhir, A ;
Gerard, M ;
Mulchandani, AK ;
Malhotra, BD .
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2001, 96 (1-3) :249-257
[10]   Conducting Langmuir-Blodgett films of polyaniline: Fabrication and charge transport properties [J].
Granholm, P ;
Paloheimo, J ;
Stubb, H .
SYNTHETIC METALS, 1997, 84 (1-3) :783-784
123