Challenging the Limit: NMR Assignment of a 31 kDa Helical Membrane Protein

被引：13

作者：

Huang, Chengdong ^{[1
]}

Mohanty, Smita ^{[1
]}

机构：

[1] Auburn Univ, Dept Chem & Biochem, Auburn, AL 36849 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2010年 / 132卷 / 11期

关键词：

YEAST OLIGOSACCHARYL TRANSFERASE; SECONDARY STRUCTURE; ESCHERICHIA-COLI; SPECTROSCOPY; SUBUNIT; COMPLEX; STT3; C-13; CRYSTALLIZATION; IDENTIFICATION;

D O I：

10.1021/ja100078z

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Structural determination of membrane proteins by NMR spectroscopy remains a challenge, especially for helical membrane proteins. Here we report the NMR assignment and secondary structure of a 31 kDa helical membrane protein, the C-terminal domain of Stt3p. The C-terminal domain of Stt3p has been proposed to be the catalytic domain of yeast oligosaccharyl transferase (OT), a multisubunit membrane-associated enzyme complex catalyzing N-glycosylation, which is an essential and highly conserved protein modification. NMR assignment is the First critical step in the determination of the high-resolution solution structure and further structure-function studies.

引用

页码：3662 / +

页数：4

共 28 条

[1] Structure of outer membrane protein A transmembrane domain by NMR spectroscopy [J].

Arora, A ;

Abildgaard, F ;

Bushweller, JH ;

Tamm, LK .

NATURE STRUCTURAL BIOLOGY, 2001, 8 (04) :334-338

[2] Isoaspartate in peptides and proteins: formation, significance, and analysis [J].

Aswad, DW ;

Paranandi, MV ;

Schurter, BT .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 2000, 21 (06) :1129-1136

[3] Engineering N-linked protein glycosylation with diverse O antigen lipopolysaccharide structures in Escherichia coli [J].

Feldman, MF ;

Wacker, M ;

Hernandez, M ;

Hitchen, PG ;

Marolda, CL ;

Kowarik, M ;

Morris, HR ;

Dell, A ;

Valvano, MA ;

Aebi, M .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (08) :3016-3021

[4] NMR structure of the integral membrane protein OmpX [J].

Fernández, C ;

Hilty, C ;

Wider, G ;

Güntert, P ;

Wüthrich, K .

JOURNAL OF MOLECULAR BIOLOGY, 2004, 336 (05) :1211-1221

[5] Recent developments in membrane-protein structural genomics [J].

Gao, FP ;

Cross, TA .

GENOME BIOLOGY, 2005, 6 (13)

[6] Solution-state NMR spectroscopy of a seven-helix transmembrane protein receptor: Backbone assignment, secondary structure, and dynamics [J].

Gautier, Antoine ;

Kirkpatrick, John P. ;

Nietlispach, Daniel .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2008, 47 (38) :7297-7300

[7] Chemoenzymatic synthesis of glycopeptides with PgIB, a bacterial oligosaccharyl transferase from Campylobacter jejuni [J].

Glover, KJ ;

Weerapana, E ;

Numao, S ;

Imperiali, B .

CHEMISTRY & BIOLOGY, 2005, 12 (12) :1311-1316

[8] A Novel Method of Production and Biophysical Characterization of the Catalytic Domain of Yeast Oligosaccharyl Transferase [J].

Huang, Chengdong ;

Mohanty, Smita ;

Banerjee, Monimoy .

BIOCHEMISTRY, 2010, 49 (06) :1115-1126

[9] Solution structure and dynamics of the outer membrane enzyme PagP by NMR [J].

Hwang, PM ;

Choy, WY ;

Lo, EI ;

Chen, L ;

Forman-Kay, JD ;

Raetz, CRH ;

Privé, GG ;

Bishop, RE ;

Kay, LE .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (21) :13560-13565

[10] Structure-guided identification of a new catalytic motif of oligosaccharyltransferase [J].

Igura, Mayumi ;

Maita, Nobuo ;

Kamishikiryo, Jun ;

Yamada, Masaki ;

Obita, Takayuki ;

Maenaka, Katsumi ;

Kohda, Daisuke .

EMBO JOURNAL, 2008, 27 (01) :234-243

← 1 2 3 →