Ion-Specific Effects on the Interaction between Fibronectin and Negatively Charged Mica Surfaces

被引：27
作者：
Heath, Matthew D. [1 ,3 ]
Henderson, Brian [2 ]
Perkin, Susan [1 ]
机构：
[1] UCL, Dept Chem, London WC1H 0AJ, England
[2] UCL, Eastman Dent Inst, Div Microbial Dis, London WC1X 8LD, England
[3] UCL, Dept Struct & Mol Biol, London WC1E 6BT, England
来源：
LANGMUIR | 2010年 / 26卷 / 08期
基金：
英国惠康基金;
关键词：
STRENGTH; PH; CONFORMATIONS; MICROSCOPY; ADSORPTION; INTEGRINS; PROTEINS; MOLECULE; SHAPE;
D O I：
10.1021/la100678n
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Atomic force microscopy (A FM) imaging and subsequent image analysis have been used to measure the ion-specific and ionic strength elects on the adsorption of fibronectin to mica surfaces in buffer solution. Increasing the concentration of monovalent Na+ salt solutions is shown to cause a transition from tightly aggregated and "string-of-heads" structures on the mica surface to well dispersed single-molccule adsorption. Studying the effect of two divalent salts. Ni2- and Ca2+, reveals a dramatic enhancement of fibronectin adsorption to mica in hurler solutions containing Ni2+, hut not for Ca2+. The origin of this ion-specific effect is discussed.
引用
收藏
页码：5304 / 5308
页数：5
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