Screening and characterization of human single-chain Fv antibody against β-amyloid peptide 40

被引：13
|
作者：
Cai, J
Wang, SZ [1 ]
Zhong, YW
Cheng, J
Ji, ZJ
Sheng, SL
Zhang, C
机构：
[1] Beijing Union Med Coll Hosp, Dept Nucl Med, Beijing 100730, Peoples R China
[2] Chinese Acad Med Sci, Beijing 100730, Peoples R China
[3] 302 Hosp PLA, Inst Infect Dis, Gene Therapy Res Ctr, Beijing 100039, Peoples R China
[4] Capital Univ Med Sci, Xuanwu Hosp, Beijing Res Lab Brain Aging, Beijing 100053, Peoples R China
来源：
NEUROREPORT | 2003年 / 14卷 / 02期
关键词：
Alzheimer's disease; amyloid-beta pepticle; phage display; single-chain antibody;
D O I：
10.1097/00001756-200302100-00022
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Expression of single-chain variable fragment antibody on the surface of filamentous bacteriophage is widely used to make antibodies with pre-defined specificities. Using direct selection on solid phase-bound amyloid-beta 40 peptide, wich is a main pathogenic feature of Alzheimer's disease, we obtained single-chain Fv antibody with specific binding activity. The binding epitope of the antibody is located between amino acids 1 and 16 of the peptide antigen. DNA sequencing showed that the gene coding for the single chain Fv antibody consists of 768 bp, and the complementarity-determining regions were deduced.
引用
收藏
页码：265 / 268
页数：4
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