Emergence of CD43-Expressing Hematopoietic Progenitors from Human Induced Pluripotent Stem Cells

被引:21
作者
Kessel, Katharina U. [1 ]
Bluemke, Anika [1 ]
Schoeler, Hans R. [1 ,2 ]
Zaehres, Holm [1 ,3 ]
Schlenke, Peter [4 ]
Dorn, Isabel [4 ]
机构
[1] Max Planck Inst Mol Biomed, Dept Cell & Dev Biol, D-48149 Munster, Germany
[2] Westphalian Wilhelms Univ Munster, Fac Med, Munster, Germany
[3] Ruhr Univ Bochum, Fac Med, Dept Anat & Mol Embryol, Bochum, Germany
[4] Med Univ Graz, Dept Blood Grp Serol & Transfus Med, Graz, Austria
关键词
Hematopoietic stem cells; Induced pluripotent stem cells; Hematopoiesis; Embryoid body; GENERATION; DIFFERENTIATION; EXPANSION; CD34; LEUKOSIALIN; EXPRESSION;
D O I
10.1159/000477357
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background: The ex vivo generation of human hematopoietic stem cells (HSCs) with long-term repopulating capacity and multi-lineage differentiation potential represents the holy grail of hematopoiesis research. In principle, human induced pluripotent stem cells (hiPSCs) provide the tool for both studying molecular mechanisms of hematopoietic development and the ex vivo production of 'true' HSCs for transplantation purposes and lineage-specific cells, e.g. red blood cells, for transfusion purposes. CD43-expressing cells have been reported as the first hematopoietic cells during development, but whether or not these possess multilineage differentiation and long-term engraftment potential is incompletely understood. Methods: We performed ex vivo generation of hematopoietic cells from hiPSCs using an embryoid body(EB)-based, xeno-product-free differentiation protocol. We investigated the multilineage differentiation potential of different FACS-sorted CD43-expressing cell subsets by colony-forming assays in semisolid media. Further, erythroid differentiation was investigated in more detail using established protocols. Results: By using CD43, we were able to measure hematopoietic induction efficiency during hiPSC-derived EB differentiation. Further, we determined CD43+ cells as the cell population of origin for in vitro erythropoiesis. Furthermore, colony formation demonstrates that the multipotent hematopoietic stem and progenitor cell fraction is particulary enriched in the CD43(hi) CD45+ population. (C) 2017 S. Karger GmbH, Freiburg
引用
收藏
页码:143 / 150
页数:8
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