Microbiota Supplementation with Bifidobacterium and Lactobacillus Modifies the Preterm Infant Gut Microbiota and Metabolome: An Observational Study

被引：143

作者：

Alcon-Giner, Cristina ^{[1
]}

Dalby, Matthew J. ^{[1
]}

Caim, Shabhonam ^{[1
]}

Ketskemety, Jennifer ^{[1
]}

Shaw, Alex ^{[2
]}

Sim, Kathleen ^{[2
]}

Lawson, Melissa A. E. ^{[1
]}

Kiu, Raymond ^{[1
]}

Leclaire, Charlotte ^{[1
]}

Chalklen, Lisa ^{[1
]}

Kujawska, Magdalena ^{[1
]}

Mitra, Suparna ^{[1
,3
]}

Fardus-Reid, Fahmina ^{[4
]}

Belteki, Gustav ^{[5
]}

McColl, Katherine ^{[6
]}

Swann, Jonathan R. ^{[4
]}

Kroll, J. Simon ^{[2
]}

Clarke, Paul ^{[6
,7
]}

Hall, Lindsay J. ^{[1
,7
,8
,9
]}

机构：

[1] Norwich Res Pk, Quadram Inst Biosci, Gut Microbes & Hlth, Norwich, Norfolk, England

[2] Imperial Coll London, Dept Med, Sect Pediat, London, England

[3] Univ Leeds, Leeds Inst Med Res, Leeds, W Yorkshire, England

[4] Imperial Coll London, Fac Med, Dept Surg & Canc, London, England

[5] Cambridge Univ Hosp NHS Fdn Trust, Rosie Hosp, Neonatal Intens Care Unit, Cambridge, England

[6] Norfolk & Norwich Univ Hosp, Neonatal Intens Care Unit, Norwich, Norfolk, England

[7] Univ East Anglia, Norwich Med Sch, Norwich, Norfolk, England

[8] Tech Univ Munich, Sch Life Sci, Chair Intestinal Microbiome, Freising Weihenstephan, Germany

[9] Tech Univ Munich, ZIEL Inst Food & Hlth, Freising Weihenstephan, Germany

来源：

CELL REPORTS MEDICINE | 2020年 / 1卷 / 05期

基金：

英国惠康基金; 英国生物技术与生命科学研究理事会;

关键词：

NECROTIZING ENTEROCOLITIS; PROBIOTICS; COLONIZATION; TRANSMISSION; IMPACT; TOOL;

D O I：

10.1016/j.xcrm.2020.100077

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Supplementation with members of the early-life microbiota as "probiotics" is increasingly used in attempts to beneficially manipulate the preterm infant gut microbiota. We performed a large observational longitudinal study comprising two preterm groups: 101 infants orally supplemented with Bifidobacterium and Lactobacillus (Bif/Lacto) and 133 infants non-supplemented (control) matched by age, sex, and delivery method. 16S rRNA gene profiling on fecal samples (n = 592) showed a predominance of Bifidobacterium and a lower abundance of pathobionts in the Bif/Lacto group. Metabolomic analysis showed higher fecal acetate and lactate and a lower fecal pH in the Bif/Lacto group compared to the control group. Fecal acetate positively correlated with relative abundance of Bifidobacterium, consistent with the ability of the supplemented Bifidobacterium strain to metabolize human milk oligosaccharides into acetate. This study demonstrates that microbiota supplementation is associated with a Bifidobacterium-dominated preterm microbiota and gastrointestinal environment more closely resembling that of full-term infants.

引用

页数：21

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