Effect of superoxide dismutase gene inactivation on virulence of Pseudomonas aeruginosa PAO1 toward the silkworm, Bombyx mori

被引：39
作者：
Iiyama, Kazuhiro [1 ]
Chieda, Yuuka
Lee, Jae Man
Kusakabe, Takahiro
Yasunaga-Aoki, Chisa
Shimizu, Susumu
机构：
[1] Kyushu Univ, Grad Sch, Fac Agr, Inst Biol,Lab Insect Pathol & Microbial Control, Fukuoka 8128581, Japan
[2] Kyushu Univ, Grad Sch, Fac Agr, Lab Silkworm Sci, Fukuoka 8128581, Japan
[3] Japan Soc Promot Sci, Tokyo, Japan
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2007年 / 73卷 / 05期
关键词：
D O I：
10.1128/AEM.00981-06
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
To investigate the role of superoxide dismutase (SOD) in virulence against the silkworm, Bombyx mori, mutants of Pseudomonas aeruginosa PAO1 lacking manganese-SOD (PAO1sodM), iron-SOD (PAO1sodB), or both (PAO1sodMB) were generated. The mutants were injected into the hemocoel of B. mori. The virulence decreased in the order PAO1 = PAO1sodM > PAO1sodB > PAO1sodMB. In particular, PAO1sodMB was avirulent at a dose of 10(5) cells or less. The sod double mutant PAO1sodMB was then complemented with either pSodM or pSodB in trans. In both the complemented strains, the virulence was partially restored. Of the two plasmids, pSodB contributed more to the virulence of P. aeruginosa against B. mori. The results of growth in B. mori hemolymph broth and microscopic analysis suggested that a longer lag phase and superoxide sensitivity correlated with decreased virulence in sod mutants. In conclusion, the SODs are required for full virulence of P. aeruginosa against B. mori and Fe-SOD is more important than Mn-SOD in the infection process.
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页码：1569 / 1575
页数：7
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