A New Primer Set to Amplify the Mitochondrial Cytochrome C Oxidase Subunit I (COI) Gene in the DHA-Rich Microalgae, the Genus Aurantiochytrium

被引：3
|
作者：
Nishitani, Goh [1 ]
Yoshida, Masaki [2 ]
机构：
[1] Tohoku Univ, Grad Sch Agr Sci, Aoba Ku, Aoba 468-1, Sendai, Miyagi 9800845, Japan
[2] Univ Tsukuba, Fac Life & Environm Sci, 1-1-1 Tennodai, Tsukuba, Ibaraki 3058572, Japan
来源：
MICROBES AND ENVIRONMENTS | 2018年 / 33卷 / 02期
关键词：
Aurantiochytrium; mitochondrial primer; COI; THRAUSTOCHYTRIDS; DNA;
D O I：
10.1264/jsme2.ME17145
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
This study was performed in order to develop a primer set for mitochondria! cytochrome c oxidase subunit I (COI) in the DHA-rich microalgae of the genus Aurantiochytrium. The performance of the primer set was tested using 12 Aurantiochytrium strains and other thraustochytrid species. There were no genetic polymorphisms in the mitochondria! sequences from the Aurantiochytrium strains, in contrast to the nuclear 18S rRNA gene sequence. This newly developed primer set amplified sequences from Aurantiochytrium and closely related genera, and may be useful for species identification and clarifying the genetic diversity of Aurantiochytrium in the field.
引用
收藏
页码：227 / 229
页数：3
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