Metabolomic Characterization of a cf. Neolyngbya Cyanobacterium from the South China Sea Reveals Wenchangamide A, a Lipopeptide with In Vitro Apoptotic Potential in Colon Cancer Cells

被引：8

作者：

Ding, Lijian ^{[1
,2
]}

Bar-Shalom, Rinat ^{[3
]}

Aharonovich, Dikla ^{[2
]}

Kurisawa, Naoaki ^{[4
]}

Patial, Gaurav ^{[1
]}

Li, Shuang ^{[1
]}

He, Shan ^{[1
]}

Yan, Xiaojun ^{[1
]}

Iwasaki, Arihiro ^{[4
,5
]}

Suenaga, Kiyotake ^{[4
,5
]}

Zhu, Chengcong ^{[5
]}

Luo, Haixi ^{[5
]}

Tian, Fuli ^{[5
]}

Fares, Fuad ^{[3
]}

Naman, C. Benjamin ^{[1
,5
]}

Luzzatto-Knaan, Tal ^{[2
]}

机构：

[1] Ningbo Univ, Coll Food & Pharmaceut Sci, Dept Marine Pharm, Li Dak Sum Yip Yio Chin Kenneth Li Marine Biophar, Ningbo 315800, Peoples R China

[2] Univ Haifa, Leon H Charney Sch Marine Sci, Dept Marine Biol, IL-31905 Haifa, Israel

[3] Univ Haifa, Fac Life Sci, Dept Human Biol, IL-31905 Haifa, Israel

[4] Keio Univ, Dept Chem, Kohoku Ku, 3-14-1 Hiyoshi, Yokohama, Kanagawa 2238522, Japan

[5] Hainan Vocat Univ Sci & Technol, Key Lab Med & Edible Plant Resources Hainan Prov, Haikou 571126, Hainan, Peoples R China

来源：

MARINE DRUGS | 2021年 / 19卷 / 07期

基金：

中国国家自然科学基金;

关键词：

metabolomics; secondary metabolites; natural products; cyanobacteria; Neolyngbya; anticancer; drug discovery; South China Sea; wenchangamide; NATURAL-PRODUCTS; MOLECULAR NETWORKING; OSCILLATORIALES; DISCOVERY;

D O I：

10.3390/md19070397

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Metabolomics can be used to study complex mixtures of natural products, or secondary metabolites, for many different purposes. One productive application of metabolomics that has emerged in recent years is the guiding direction for isolating molecules with structural novelty through analysis of untargeted LC-MS/MS data. The metabolomics-driven investigation and bioassay-guided fractionation of a biomass assemblage from the South China Sea dominated by a marine filamentous cyanobacteria, cf. Neolyngbya sp., has led to the discovery of a natural product in this study, wenchangamide A (1). Wenchangamide A was found to concentration-dependently cause fast-onset apoptosis in HCT116 human colon cancer cells in vitro (24 h IC50 = 38 mu M). Untargeted metabolomics, by way of MS/MS molecular networking, was used further to generate a structural proposal for a new natural product analogue of 1, here coined wenchangamide B, which was present in the organic extract and bioactive sub-fractions of the biomass examined. The wenchangamides are of interest for anticancer drug discovery, and the characterization of these molecules will facilitate the future discovery of related natural products and development of synthetic analogues.

引用

页数：18

共 51 条

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Anagnostidis Konstantinos, 2001, Preslia (Prague), V73, P359

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