Method for measuring gap junctional intercellular communication by fluorescence recovery after photobleaching

被引：0
|
作者：
Luo, SQ [1 ]
Mo, YY
Seguchi, H
机构：
[1] First Mil Med Univ, Dept Mol Genet & Cell Biol, Guangzhou, Peoples R China
[2] Kochi Med Sch, Dept Anat & Cell Biol, Kochi 783, Japan
来源：
ACTA HISTOCHEMICA ET CYTOCHEMICA | 1997年 / 30卷 / 5-6期
关键词：
FRAP; communication; gap junction;
D O I：
10.1267/ahc.30.575
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The present study was designed to establish a method for measuring cultured neuroglial gap junctional intercellular communication (GJIC) of rat cerebral cortices by fluorescence recovery after photobleaching (FRAP) analysis technique. As a result of the managements, such as cell culture, fluorescence staining, fluorescence excitement, laser scanning and computer analysis et al., fluorescence inside all selected cells recovered in different degrees, i.e., fluorescence relative intensities in the 1st-5th cell raised 14%, 28%, 43%, 17.5% and 12.5% respectively after scanning 15.4 min, the mean value of recovery was 18.57 +/- 10.06% and the mean rate of fluorescence recovery was 1.223 +/- 0.785%/min for three samples (n=14 cells). The result showed that the FRAP is an ideal method for measuring GJIC.
引用
收藏
页码：575 / 578
页数：4
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