Development of albumin microspheres containing Sp H1-DNA complexes: A novel gene delivery system

被引：4

作者：

Okoroukwu, Odilia N. ^{[1
]}

Green, George R. ^{[1
]}

D'Souza, Martin J. ^{[1
]}

机构：

[1] Mercer Univ, Coll Pharm & Hlth Sci, Dept Pharmaceut Sci, Atlanta, GA 30341 USA

来源：

JOURNAL OF MICROENCAPSULATION | 2010年 / 27卷 / 02期

关键词：

DNA delivery; gene transfer; albumin microsphere; sea urchin sperm histone H1; MARROW-DERIVED CELLS; PLASMID DNA; DRUG-DELIVERY; VIRUS; PHOSPHORYLATION; IMMUNIZATION; VACCINATION; INDUCTION; STABILITY; PROTEINS;

D O I：

10.3109/02652040903052028

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

This study investigated the stability and transfection efficiency of plasmid DNA (pDNA) and sea urchin sperm histone H1 (Sp H1) complexes embedded in albumin microsphere formulations. Sp H1 increased the stability and transfection efficiency of pDNA, while providing a favourable sustained pDNA release profile. Encapsulating Sp H1-complexed pDNA into albumin microspheres further protected the pDNA from physical stress and heparin treatment. When compared with free pDNA encapsulated in albumin microspheres, the Sp H1-pDNA microsphere formulations exhibited decreased hydrophilicity, slower pDNA release profiles, protection against heparin-induced degradation of embedded pDNA and increased stability against physical stress. These results indicate that complex formation of pDNA with Sp H1 facilitates intracellular DNA transfer and that albumin microspheres-Sp H1-pDNA gene delivery formulations are suitable for controlled-release delivery of pDNA while offering protection of the pDNA from degradation and maintaining pDNA biological activity.

引用

页码：142 / 149

页数：8