Effect of tenascin and fibronectin on the migration of human corneal fibroblasts

被引：19

作者：

Schmidinger, G ^{[1
]}

Hanselmayer, G ^{[1
]}

Pieh, S ^{[1
]}

Lackner, B ^{[1
]}

Kaminski, S ^{[1
]}

Ruhswurm, I ^{[1
]}

Skorpik, C ^{[1
]}

机构：

[1] Univ Vienna, Sch Med, Dept Ophthalmol, A-1090 Vienna, Austria

来源：

JOURNAL OF CATARACT AND REFRACTIVE SURGERY | 2003年 / 29卷 / 02期

关键词：

D O I：

10.1016/S0886-3350(02)01609-7

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Purpose: To investigate the effect of fibronectin and tenascin on the migration of corneal fibroblasts. Setting. Department of Ophthalmology, University of Vienna, Medical School, Vienna, Austria. Methods: Using human corneal fibroblasts, a monolayer migration assay was performed to measure corneal fibroblast movement. The migration on collagen 1, fibronectin, and tenascin with and without transforming growth factor (TGF)-alpha/fibroblast growth factor (FGF)-2 stimulation and the effect of soluble tenascin and fibronectin on corneal fibroblast migration on collagen-I-coated wells were investigated. Results: The cytokines TGF-alpha and FGF-2 stimulated migrational activity of corneal stromal cells in a dose-dependent manner, reaching the maximum effect at 100 ng/mL and 10 ng/mL, respectively. The migration of corneal fibroblasts on fibronectin was significantly higher (P < .05) than the migration on collagen I. Transforming growth factor-α and FGF-2 increased radial cell displacement independent of the provided matrix composition-Tenascin had a negative effect on corneal fibroblast adhesion/migration in this in vitro model. Conclusion: Fibronectin and tenascin influenced corneal fibroblast migration and adhesion, respectively, and may play a role in stromal cell movement during. wound healing. The cytokines TGF-α and FGF-2 had an additive effect on corneal fibroblast migration on a fibronectin matrix.

引用

页码：354 / 360

页数：7

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