Effects of ammonium and nitrite on communities and populations of ammonia-oxidizing bacteria in laboratory-scale continuous-flow reactors

被引:55
作者
Limpiyakorn, Tawan
Kurisu, Futoshi
Sakamoto, Yoriko
Yagi, Osami
机构
[1] Univ Tokyo, Grad Sch Engn, Dept Urban Engn, Tokyo, Japan
[2] Univ Tokyo, Grad Sch Engn, Res Ctr Water Environm Technol, Tokyo, Japan
关键词
ammonium; ammonia-oxidizing bacteria; continuous-flow reactor; nitrite; real-time PCR; 16S rRNA gene sequences;
D O I
10.1111/j.1574-6941.2007.00307.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
This study investigated the effects of ammonium and nitrite on ammonia-oxidizing bacteria (AOB) from an activated sludge process in laboratory-scale continuous-flow reactors. AOB communities were analyzed using specific PCR followed by denaturing gel gradient electrophoresis, cloning and sequencing of the 16S rRNA gene, and AOB populations were quantified using real-time PCR. To study the effect of ammonium, activated sludge from a sewage treatment system was enriched in four reactors receiving inorganic medium containing four different ammonium concentrations (2, 5, 10 and 30 mM NH4+-N). One of several sequence types of the Nitrosomonas oligotropha cluster predominated in the reactors with lower ammonium loads (2, 5 and 10 mM NH4+-N), whereas Nitrosomonas europaea was the dominant AOB in the reactor with the highest ammonium load (30 mM NH4+-N). The effect of nitrite was studied by enriching the enriched culture possessing both N. oligotropha and N. europaea in four reactors receiving 10-mM-ammonium inorganic medium containing four different nitrite concentrations (0, 2, 12 and 22 mM NO2--N). Nitrosomonas oligotropha comprised the majority of AOB populations in the reactors without nitrite accumulation (0 and 2 mM NO2--N), whereas N. europaea was in the majority in the 12- and 22-mM NO2--N reactors, in which nitrite concentrations were 2.1-5.7 mM (30-80 mg N L-1).
引用
收藏
页码:501 / 512
页数:12
相关论文
共 35 条
[1]   RFLP of rRNA genes and sequencing of the 16S-23S rDNA intergenic spacer region of ammonia-oxidizing bacteria:: a phylogenetic approach [J].
Aakra, Å ;
Utåker, JB ;
Nes, IF .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1999, 49 :123-130
[2]   Molecular microbial ecology of nitrification in an activated sludge process treating refinery wastewater [J].
Ballinger, SJ ;
Head, IM ;
Curtis, TP ;
Godley, AR .
WATER SCIENCE AND TECHNOLOGY, 1998, 37 (4-5) :105-108
[3]   GROWTH AND OXIDATION-KINETICS OF 3 GENERA OF AMMONIA OXIDIZING NITRIFIERS [J].
BELSER, LW ;
SCHMIDT, EL .
FEMS MICROBIOLOGY LETTERS, 1980, 7 (03) :213-216
[4]   Continuous culture enrichments of ammonia-oxidizing bacteria at low ammonium concentrations [J].
Bollmann, A ;
Laanbroek, HJ .
FEMS MICROBIOLOGY ECOLOGY, 2001, 37 (03) :211-221
[5]   Cultivation-independent, semiautomatic determination of absolute bacterial cell numbers in environmental samples by fluorescence in situ hybridization [J].
Daims, H ;
Ramsing, NB ;
Schleifer, KH ;
Wagner, M .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (12) :5810-5818
[6]   Quantification of Nitrosomonas oligotropha-like ammonia-oxidizing bacteria and Nitrospira spp. from full-scale wastewater treatment plants by competitive PCR [J].
Dionisi, HM ;
Layton, AC ;
Harms, G ;
Gregory, IR ;
Robinson, KG ;
Sayler, GS .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2002, 68 (01) :245-253
[7]   Community structure and activity dynamics of nitrifying bacteria in a phosphate-removing biofilm [J].
Gieseke, A ;
Purkhold, U ;
Wagner, M ;
Amann, R ;
Schramm, A .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (03) :1351-1362
[8]   Real-time PCR quantification of nitrifying bacteria in a municipal wastewater treatment plant [J].
Harms, G ;
Layton, AC ;
Dionisi, HM ;
Gregory, IR ;
Garrett, VM ;
Hawkins, SA ;
Robinson, KG ;
Sayler, GS .
ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2003, 37 (02) :343-351
[9]  
Hellinga C, 1998, WATER SCI TECHNOL, V37, P135, DOI 10.2166/wst.1998.0350
[10]   Quantification of ammonia-oxidizing bacteria in arable soil by real-time PCR [J].
Hermansson, A ;
Lindgren, PE .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (02) :972-976