The effects of Mycoplasma hyopneumoniae on porcine circovirus type 2 replication in vitro PK-15 cells

被引：10

作者：

Wang, Haiyan ^{[1
,2
]}

Feng, Zhixin ^{[1
,2
]}

Wu, Yuzi ^{[1
]}

Wei, Yanna ^{[1
]}

Gan, Yuan ^{[1
]}

Hua, Lizhong ^{[1
]}

Li, Bin ^{[1
]}

Wang, Xiaomin ^{[1
]}

Liu, Maojun ^{[1
]}

Xiong, Qiyan ^{[1
]}

Shao, Guoqing ^{[1
,2
]}

机构：

[1] Jiangsu Acad Agr Sci, Inst Vet Med, Key Lab Vet Biol Engn & Technol, Minist Agr,Natl Ctr Engn Res Vet Bioprod, Nanjing 210014, Jiangsu, Peoples R China

[2] Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China

来源：

RESEARCH IN VETERINARY SCIENCE | 2016年 / 105卷

关键词：

Porcine circovirus type 2; Mycoplasma hyopneumoniae; Co-infection; Replication; MULTISYSTEMIC WASTING SYNDROME; RESPIRATORY SYNDROME VIRUS; EXPERIMENTAL REPRODUCTION; EXPERIMENTAL-INFECTION; ENZOOTIC PNEUMONIA; CONVENTIONAL PIGS; SYNDROME PMWS; HOST-CELLS; DISEASE; PORCINE-CIRCOVIRUS-2;

D O I：

10.1016/j.rvsc.2016.01.015

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Porcine circovirus type 2 (PCV2) is the causative agent of postweaning multisystemic wasting syndrome (PMWS). Mycoplasma hyopneumoniae (Mhp) is a very well-known co-factor that potentially enhances PCV2 replication and thus the development of PMWS. However, co-infection with Mhp and PCV2 in vivo under different conditions can produce divergent clinical signs and lesions. In this study, PCV2 replication could be enhanced by subsequent co-inoculation with Mhp (PCV2 + Mhp) in a time and dose dependent method, but not by prior (Mhp + PCV2) or simultaneous (Mhp/PCV2) co-inoculation. Furthermore, different magnitudes of PCV2-infected cells, varying from 150% +/- 14% to 351% +/- 28%, were detected when co-infected with different Mhp strains. The relative percentage of PCV2-infected cells greatly decreased from 351%+/- 28 to 141%+/- 8 when the Mhp strain was treated with UV light for 12 h. These results offer the evidences to better understand the complex clinical syndromes in Mhp/PCV2 co-infection cases, and the occurrence of PMWS. (C) 2016 Published by Elsevier Ltd.

引用

页码：56 / 61

页数：6

共 43 条

[21] Hsp70 positively regulates porcine circovirus type 2 replication in vitro [J].

Liu, Jie ;

Bai, Juan ;

Zhang, Lili ;

Jiang, Zhihua ;

Wang, Xianwei ;

Li, Yufeng ;

Jiang, Ping .

VIROLOGY, 2013, 447 (1-2) :52-62

[22] The ORF3 protein of porcine circovirus type 2 is involved in viral pathogenesis in vivo [J].

Liu, Jue ;

Chen, Isabelle ;

Du, Qinayun ;

Chua, Huikheng ;

Kwang, Jimmy .

JOURNAL OF VIROLOGY, 2006, 80 (10) :5065-5073

[23] Enzootic pneumonia in pigs [J].

Maes, D ;

Verdonck, M ;

Deluyker, H ;

deKruif, A .

VETERINARY QUARTERLY, 1996, 18 (03) :104-109

[24] Interactions of highly and low virulent Mycoplasma hyopneumoniae isolates with the respiratory tract of pigs [J].

Meyns, T. ;

Maes, D. ;

Calus, D. ;

Ribbens, S. ;

Dewulf, J. ;

Chiers, K. ;

de Kruif, A. ;

Cox, E. ;

Decostere, A. ;

Haesebrouck, F. .

VETERINARY MICROBIOLOGY, 2007, 120 (1-2) :87-95

[25] Porcine circovirus 2 uses heparan sulfate and chondroitin sulfate B glycosaminoglycans as receptors for its attachment to host cells [J].

Misinzo, G ;

Delputte, PL ;

Meerts, P ;

Lefebvre, DJ ;

Nauwynck, HJ .

JOURNAL OF VIROLOGY, 2006, 80 (07) :3487-3494

[26] Inhibition of endosome-lysosome system acidification enhances porcine circovirus 2 infection of porcine epithelial cells [J].

Misinzo, Gerald ;

Delputte, Peter L. ;

Nauwynck, Hans J. .

JOURNAL OF VIROLOGY, 2008, 82 (03) :1128-1135

[27] Emerging picture of host chaperone and cyclophilin roles in RNA virus replication [J].

Nagy, P. D. ;

Wang, R. Y. ;

Pogany, J. ;

Hafren, A. ;

Makinen, K. .

VIROLOGY, 2011, 411 (02) :374-382

[28] Experimental reproduction of postweaning multisystemic wasting syndrome in pigs by dual infection with Mycoplasma hyopneamoniae and porcine circovirus type 2 [J].

Opriessnig, T ;

Thacker, EL ;

Yu, S ;

Fenaux, M ;

Meng, XJ ;

Halbur, PG .

VETERINARY PATHOLOGY, 2004, 41 (06) :624-640

[29] Porcine circovirus type 2 (PCV2) vaccination is effective in reducing disease and PCV2 shedding in semen of boars concurrently infected with PCV2 and Mycoplasma hyopneumoniae [J].

Opriessnig, T. ;

Madson, D. M. ;

Schalk, S. ;

Brockmeier, S. ;

Shen, H. G. ;

Beach, N. M. ;

Meng, X. J. ;

Baker, R. B. ;

Zanella, E. L. ;

Halbur, P. G. .

THERIOGENOLOGY, 2011, 76 (02) :351-360

[30] Experimental infection of 3-week-old conventional colostrum-fed pigs with porcine circovirus type 2 and porcine parvovirus [J].

Ostanello, F ;

Caprioli, A ;

Di Francesco, A ;

Battilani, M ;

Sala, G ;

Sarli, G ;

Mandrioli, L ;

McNeilly, F ;

Allan, GM ;

Prosperi, S .

VETERINARY MICROBIOLOGY, 2005, 108 (3-4) :179-186

← 1 2 3 4 5 →