The effects of Mycoplasma hyopneumoniae on porcine circovirus type 2 replication in vitro PK-15 cells

被引：10

作者：

Wang, Haiyan ^{[1
,2
]}

Feng, Zhixin ^{[1
,2
]}

Wu, Yuzi ^{[1
]}

Wei, Yanna ^{[1
]}

Gan, Yuan ^{[1
]}

Hua, Lizhong ^{[1
]}

Li, Bin ^{[1
]}

Wang, Xiaomin ^{[1
]}

Liu, Maojun ^{[1
]}

Xiong, Qiyan ^{[1
]}

Shao, Guoqing ^{[1
,2
]}

机构：

[1] Jiangsu Acad Agr Sci, Inst Vet Med, Key Lab Vet Biol Engn & Technol, Minist Agr,Natl Ctr Engn Res Vet Bioprod, Nanjing 210014, Jiangsu, Peoples R China

[2] Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China

来源：

RESEARCH IN VETERINARY SCIENCE | 2016年 / 105卷

关键词：

Porcine circovirus type 2; Mycoplasma hyopneumoniae; Co-infection; Replication; MULTISYSTEMIC WASTING SYNDROME; RESPIRATORY SYNDROME VIRUS; EXPERIMENTAL REPRODUCTION; EXPERIMENTAL-INFECTION; ENZOOTIC PNEUMONIA; CONVENTIONAL PIGS; SYNDROME PMWS; HOST-CELLS; DISEASE; PORCINE-CIRCOVIRUS-2;

D O I：

10.1016/j.rvsc.2016.01.015

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Porcine circovirus type 2 (PCV2) is the causative agent of postweaning multisystemic wasting syndrome (PMWS). Mycoplasma hyopneumoniae (Mhp) is a very well-known co-factor that potentially enhances PCV2 replication and thus the development of PMWS. However, co-infection with Mhp and PCV2 in vivo under different conditions can produce divergent clinical signs and lesions. In this study, PCV2 replication could be enhanced by subsequent co-inoculation with Mhp (PCV2 + Mhp) in a time and dose dependent method, but not by prior (Mhp + PCV2) or simultaneous (Mhp/PCV2) co-inoculation. Furthermore, different magnitudes of PCV2-infected cells, varying from 150% +/- 14% to 351% +/- 28%, were detected when co-infected with different Mhp strains. The relative percentage of PCV2-infected cells greatly decreased from 351%+/- 28 to 141%+/- 8 when the Mhp strain was treated with UV light for 12 h. These results offer the evidences to better understand the complex clinical syndromes in Mhp/PCV2 co-infection cases, and the occurrence of PMWS. (C) 2016 Published by Elsevier Ltd.

引用

页码：56 / 61

页数：6

共 43 条

[1] Experimental reproduction of severe wasting disease by co-infection of pigs with porcine circovirus and porcine parvovirus [J].