Cell-mediated immunity to porcine reproductive and respiratory syndrome virus in swine

被引:115
作者
Bautista, EM [1 ]
Molitor, TW [1 ]
机构
[1] UNIV MINNESOTA,COLL VET MED,DEPT CLIN & POPULAT SCI,ST PAUL,MN 55108
关键词
D O I
10.1089/vim.1997.10.83
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Cell-mediated immunity has been demonstrated to be a necessary component of immunity against viral infection. Methods to detect T-cell mediated immune responses to porcine reproductive and respiratory syndrome virus (PRRSV) infection were established both in vitro as lymphocyte proliferation and in vivo as delayed-type hypersensitivity response (DTH). Optimal conditions for detection of lymphocyte proliferation were determined by testing different antigen concentrations and various stimulation periods. The proliferation response to PRRSV was antigen-specific and dose-dependent. The kinetics of the T-cell proliferation response to PRRSV were analyzed after primary and secondary exposure to virus. Lymphocyte proliferation was first detected at four weeks post-infection (PI), peaked at 7 weeks PI, and declined after 11 weeks PI. The secondary response increased in magnitude. Experiments with blocking antibodies to porcine leukocyte antigens demonstrated that CD4(+) T-cells were the major effector cells in the proliferation response. The in vivo response to PRRSV was shown by detection of a dose-dependent DTH reaction in infected pigs after intradermal challenge with UV-inactivated virus. These results demonstrate that pigs generate specific T-cell responses on PRRSV infection and provide a foundation for studying their role in protection.
引用
收藏
页码:83 / 94
页数:12
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