Marinobacter gudaonensis sp nov., isolated from an oil-polluted saline soil in a Chinese oilfield

Two novel strains, SL0141361A(T) and SL014B11A, were isolated from an oil-polluted saline soil from Gudao in the coastal Shengli Oilfield, eastern China. Cells of strains SL014B61A(T) and SL014B11A were motile, Gram-negative and rod-shaped. Growth occurred at NaCl concentrations of between 0 and 15% and at temperatures of between 10 and 45 degrees C. Strain SL014B61A(T) had Q9 asthe major respiratory quinone and C16:0 (21.2 %), C18:1 omega 9c(20.3%), C16: 1 omega 7c (7.3%) and C16: 1 omega 9c (6.4%) as predominant fatty acids. The G +C content of the DNA was 57.9 mol%. Phylogenetic: analysis based on 16S rRNA gene sequences indicated that strain SL014B61A(T) belonged to the genus Marinobacter in the class Gammaproteobacteria. Strain SL014B61AT showed the highest 16S rRNA gene sequence similarity with Marinobacter bryozoorum (97.9 %) and showed 97.8 % sequence similarity to Marinobacter lipolyticus. DNA-DNA relatedness to the reference strains Marinobacter bryozoorum and Marinobacter lipolyticus was 35.5% and 33.8%, respectively. On the basis of these data, it is proposed that strains SL01413611 A T and SL014B11 A represent a novel species, Marinobacter gudaonensis sp. nov. The type strain is strain SL014B611A(T) (=DSM 18066(T) =LMG 23509(T)= CGMCC 1.6294(T)).