Baicalein pretreatment reduces liver ischemia/reperfusion injury via induction of autophagy in rats

被引：59

作者：

Liu, Anding ^{[1
,2
]}

Huang, Liang ^{[3
]}

Guo, Enshuang ^{[4
,5
]}

Li, Renlong ^{[4
,5
]}

Yang, Jiankun ^{[1
]}

Li, Anyi ^{[6
]}

Yang, Yan ^{[1
]}

Liu, Shenpei ^{[1
]}

Hu, Jifa ^{[1
]}

Jiang, Xiaojing ^{[5
]}

Dirsch, Olaf ^{[2
]}

Dahmen, Uta ^{[2
]}

Sun, Jian ^{[7
]}

机构：

[1] Huazhong Univ Sci & Technol, Tongji Hosp, Tongji Med Coll, Ctr Med Expt, 1095 Jiefang Ave, Wuhan 430030, Peoples R China

[2] Univ Jena, Dept Gen Visceral & Vasc Surg, Expt Transplantat Surg, 1 Drackendorfer Str, D-07747 Jena, Germany

[3] Huazhong Univ Sci & Technol, Tongji Hosp, Dept Blood Transfus, Tongji Med Coll, 1095 Jiefang Ave, Wuhan 430030, Peoples R China

[4] Southern Med Univ, Grad Sch, 1023 Shatai Nan Rd, Guangzhou 510515, Guangdong, Peoples R China

[5] Wuhan Gen Hosp Guangzhou Mil Command, Dept Infect Dis, 627 Wuluo Rd, Wuhan 430070, Peoples R China

[6] Huazhong Univ Sci & Technol, Tongji Hosp, Tongji Med Coll, Ctr Expt Anim, 1095 Jiefang Ave, Wuhan 430030, Peoples R China

[7] Sun Yat Sen Univ, Sun Yat Sen Mem Hosp, Dept Biliopancreat Surg, 107 Yanjiang Xi Rd, Guangzhou 510120, Guangdong, Peoples R China

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

基金：

高等学校博士学科点专项科研基金;

关键词：

ISCHEMIA-REPERFUSION INJURY; HEME OXYGENASE-1; IMPAIRED AUTOPHAGY; CELL-DEATH; KAPPA-B; PROTECTS; INHIBITION; DAMAGE; APOPTOSIS; PATHWAY;

D O I：

10.1038/srep25042

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We previously demonstrated that baicalein could protect against liver ischemia/reperfusion (I/R) injury in mice. The exact mechanism of baicalein remains poorly understood. Autophagy plays an important role in protecting against I/R injury. This study was designed to determine whether baicalein could protect against liver I/R injury via induction of autophagy in rats. Baicalein was intraperitoneally injected 1 h before warm ischemia. Pretreatment with baicalein prior to I/R insult significantly blunted I/R-induced elevations of serum aminotransferase levels and significantly improved the histological status of livers. Electron microscopy and expression of the autophagic marker LC3B-II suggested induction of autophagy after baicalein treatment. Moreover, inhibition of the baicalein-induced autophagy using 3-methyladenine (3-MA) worsened liver injury. Furthermore, baicalein treatment increased heme oxygenase (HO)-1 expression, and pharmacological inhibition of HO-1 with tin protoporphyrin IX (SnPP) abolished the baicalein-mediated autophagy and the hepatocellular protection. In primary rat hepatocytes, baicalein-induced autophagy also protected hepatocytes from hypoxia/reoxygenation injury in vitro and the beneficial effect was abrogated by 3-MA or Atg7 siRNA, respectively. Suppression of HO-1 activity by SnPP or HO-1 siRNA prevented the baicalein-mediated autophagy and resulted in increased hepatocellular injury. Collectively, these results suggest that baicalein prevents hepatocellular injury via induction of HO-1-mediated autophagy.

引用

页数：12

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