Kinetics of double strand breaks of DNA in tritiated water evaluated using single molecule observation method

被引：7

作者：

Hatano, Yuji ^{[1
]}

Konaka, Yuna ^{[2
]}

Shimoyachi, Hiroto ^{[2
]}

Kenmotsu, Takahiro ^{[3
]}

Oya, Yasuhisa ^{[4
]}

Nakamura, Hiroaki ^{[5
]}

机构：

[1] Univ Toyama, Hydrogen Isotope Res Ctr, Toyama 9308555, Japan

[2] Univ Toyama, Fac Sci, Toyama 9308555, Japan

[3] Doshisha Univ, Fac Life & Med Sci, Kyoto 6100321, Japan

[4] Shizuoka Univ, Coll Sci, Shizuoka 4228529, Japan

[5] Natl Inst Fus Sci, Toki, Gifu 5095292, Japan

来源：

FUSION ENGINEERING AND DESIGN | 2019年 / 146卷

关键词：

Tritium; beta-rays; DNA; Radiation effects; GIANT DNA;

D O I：

10.1016/j.fusengdes.2018.11.045

中图分类号：

TL [原子能技术]; O571 [原子核物理学];

学科分类号：

0827 ; 082701 ;

摘要：

Double strand breaks (DSBs) of DNA molecules in tritiated water was examined under sterilized and non-sterilized conditions using a single molecule observation method. The genome DNA of bacteriophage T4 GT7 was immersed in sterilized tritiated water (5.2 MBq/cm(3)) and non-sterilized tritiated water (4.2 MBq/cm(3)) for 1, 7 and 14 day(s). Then the length of DNA molecules was measured using a fluorescence microscope after intercalation of fluorescent dye. The dose rate was 1.4-1.7 x 10(-2) Gy/h and the dose level was 0.41-5.8 Gy. The rate of DSBs induced by beta-rays from tritium was successfully evaluated under the sterilized conditions and the value comparable with the DSB rate under gamma-ray irradiation (Noda et al., Scientific Reports 7 (2017) 8557) was obtained. The length of DNA molecules in non-sterilized tritiated water was clearly shorter than that in the sterilized tritiated water. This observation suggested that the effects of tritium was far weaker than that of microorganisms (e.g. bacteria) and impurities in water even at the tritium concentration as high as 5.2 MBq/cm(3).

引用

页码：100 / 102

页数：3

共 7 条

[1] Protective effect of ascorbic acid against double-strand breaks in giant DNA: Marked differences among the damage induced by photo-irradiation, gamma-rays and ultrasound [J].