Structural characterization of the serotype O:5 O-polysaccharide antigen of the lipopolysaccharide of Escherichia coli O:5

被引：0
|
作者：
MacLean, LL [1 ]
Perry, MB [1 ]
机构：
[1] NATL RES COUNCIL CANADA,INST BIOL SCI,OTTAWA,ON K1A 0R6,CANADA
来源：
BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE | 1997年 / 75卷 / 03期
关键词：
Escherichia coli; lipopolysaccharide; polysaccharide; O antigen; NMR;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The structure of the antigenic O-polysaccharide component of the smooth lipopolysaccharide produced by Escherichia coli serotype O:5 was investigated by composition, methylation, and periodate oxidation methods, and by 1D and 2D nuclear magnetic resonance spectroscopy. The antigenic O-chain was determined to be a high molecular weight polysaccharide composed of repeating tetrasaccharide units containing 2-acetamido-2-deoxy-D-galactose, D-galactose, D-ribose, and 3-acetamido-3,6-dideoxy-D-glucose and having the structure -[-4)-beta-D-Galp-(1-3)-alpha-D-GalpNAc-(1-4)-beta-D-Quinp3NAc-(1-3)-beta-D-Ribf-(1-]-.
引用
收藏
页码：199 / 205
页数：7
相关论文
共 50 条
  • [31] Structural identification of the lipopolysaccharide O-antigen produced by Yersinia enterocolitica serotype O:28
    Perry, MB
    MacLean, LL
    EUROPEAN JOURNAL OF BIOCHEMISTRY, 2000, 267 (09): : 2567 - 2572
  • [32] Structural studies of the O-antigen polysaccharide from Escherichia coli O115 and biosynthetic aspects thereof
    Fontana, Carolina
    Ramstrom, Kristoffer
    Weintraub, Andrej
    Widmalm, Goran
    GLYCOBIOLOGY, 2013, 23 (03) : 354 - 362
  • [33] Structural studies of the O-antigenic polysaccharide of the bovine mastitis isolate Escherichia coli serotype O174
    Duda, Katarzyna A.
    Fruth, Angelika
    Holst, Otto
    CARBOHYDRATE RESEARCH, 2013, 373 : 18 - 21
  • [34] Escherichia coli O106, a new member of a group of enteric bacteria sharing an O-polysaccharide backbone structure
    Shashkov, A. S.
    Senchenkova, S. N.
    Naumenko, O. I.
    Kalinchuk, N. A.
    Perepelov, A. V.
    Knirel, Yu. A.
    RUSSIAN CHEMICAL BULLETIN, 2018, 67 (08) : 1538 - 1541
  • [35] Structure of the O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O29
    Bushmarinov, Ivan S.
    Ovchinnikova, Olga G.
    Kocharova, Nina A.
    Toukach, Filip V.
    Torzewska, Agnieszka
    Shashkov, Alexander S.
    Knirel, Yurly A.
    Rozalski, Antoni
    CARBOHYDRATE RESEARCH, 2006, 341 (09) : 1181 - 1185
  • [36] Structure of the O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O12
    Parkhomchuk, Angelina A.
    Kocharova, Nina A.
    Bialczak-Kokot, Magdalena
    Shashkov, Alexander S.
    Chizhov, Alexander O.
    Knirel, Yuriy A.
    Rozalski, Antoni
    CARBOHYDRATE RESEARCH, 2010, 345 (09) : 1235 - 1239
  • [37] Structure of the O-polysaccharide and characterization of the O-antigen gene cluster of Salmonella enterica O53
    Perepelov, Andrei V.
    Liu, Bin
    Senchenkova, Sof'ya N.
    Shashkov, Alexander S.
    Feng, Lu
    Knirel, Yuriy A.
    Wang, Lei
    CARBOHYDRATE RESEARCH, 2011, 346 (02) : 373 - 376
  • [38] The structure of the O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O30
    Kocharova, NA
    Ovchinnikova, OG
    Torzewska, A
    Shashkov, AS
    Knirel, YA
    Rozalski, A
    CARBOHYDRATE RESEARCH, 2006, 341 (06) : 786 - 790
  • [39] Structure of the O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O33
    Ovchinnikova, Olga G.
    Shashkov, Alexander S.
    Chizhov, Alexander O.
    Moryl, Magdalena
    Rozalski, Antoni
    Knirel, Yuriy A.
    CARBOHYDRATE RESEARCH, 2014, 390 : 67 - 70
  • [40] Structure of the O-polysaccharide from the lipopolysaccharide of Providencia alcalifaciens O60
    Ovchinnikova, Olga G.
    Kocharova, Nina A.
    Parkhomchuk, Angelina A.
    Bialczak-Kokot, Magdalena
    Shashkov, Alexander S.
    Knirel, Yuriy A.
    Rozalski, Antoni
    CARBOHYDRATE RESEARCH, 2011, 346 (02) : 377 - 380
12345