Structural characterization of the serotype O:5 O-polysaccharide antigen of the lipopolysaccharide of Escherichia coli O:5

被引：0
|
作者：
MacLean, LL [1 ]
Perry, MB [1 ]
机构：
[1] NATL RES COUNCIL CANADA,INST BIOL SCI,OTTAWA,ON K1A 0R6,CANADA
来源：
BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE | 1997年 / 75卷 / 03期
关键词：
Escherichia coli; lipopolysaccharide; polysaccharide; O antigen; NMR;
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The structure of the antigenic O-polysaccharide component of the smooth lipopolysaccharide produced by Escherichia coli serotype O:5 was investigated by composition, methylation, and periodate oxidation methods, and by 1D and 2D nuclear magnetic resonance spectroscopy. The antigenic O-chain was determined to be a high molecular weight polysaccharide composed of repeating tetrasaccharide units containing 2-acetamido-2-deoxy-D-galactose, D-galactose, D-ribose, and 3-acetamido-3,6-dideoxy-D-glucose and having the structure -[-4)-beta-D-Galp-(1-3)-alpha-D-GalpNAc-(1-4)-beta-D-Quinp3NAc-(1-3)-beta-D-Ribf-(1-]-.
引用
收藏
页码：199 / 205
页数：7
相关论文
共 50 条
  • [1] The structural characterization of the O-polysaccharide antigen of the lipopolysaccharide of Escherichia coli serotype O118 and its relation to the O-antigens of Escherichia coli O151 and Salmonella enterica O47
    MacLean, Leann L.
    Liu, Yanhong
    Vinogradov, Evgeny
    Perry, Malcolm B.
    CARBOHYDRATE RESEARCH, 2010, 345 (18) : 2664 - 2669
  • [2] Structural characterization of the O-antigenic polysaccharide of Escherichia coli serotype O17 lipopolysaccharide
    Masoud, H
    Perry, MB
    BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE, 1996, 74 (02): : 241 - 248
  • [3] Characterization of the antigenic O-polysaccharide produced by Escherichia coli serotype O:70
    MacLean, Leann L.
    Perry, Malcolm B.
    CARBOHYDRATE RESEARCH, 2010, 345 (05) : 644 - 648
  • [4] Structural studies of the O-polysaccharide from the Escherichia coli O77 lipopolysaccharide
    Yildirim, H
    Weintraub, A
    Widmalm, G
    CARBOHYDRATE RESEARCH, 2001, 333 (02) : 179 - 183
  • [5] The structure of the antigenic O-polysaccharide in the lipopolysaccharide of enterohaemorrhagic Escherichia coli serotype O71:H12
    MacLean, Leann L.
    Vinogradov, Evgeny
    Perry, Malcolm B.
    BIOCHEMISTRY AND CELL BIOLOGY-BIOCHIMIE ET BIOLOGIE CELLULAIRE, 2010, 88 (03): : 439 - 444
  • [6] Structural studies on the O-polysaccharide of Escherichia coli O57
    Naumenko, Olesya I.
    Song, Jingjie
    Senchenkova, Sof'ya N.
    Jiang, Xiaohan
    Perepelov, Andrei V.
    Shashkov, Alexander S.
    Knirel, Yuriy A.
    CARBOHYDRATE RESEARCH, 2018, 465 : 1 - 3
  • [7] Structure of the O-polysaccharide of Escherichia coli O60
    Perepelov, A. V.
    Naumenko, O. I.
    Senchenkova, S. N.
    Shashkov, A. S.
    Chizhov, A. O.
    Knirel, Yu. A.
    RUSSIAN CHEMICAL BULLETIN, 2018, 67 (11) : 2131 - 2134
  • [8] Structure of the O-polysaccharide of Escherichia coli O132
    Shashkov, Alexander S.
    Zhang, Wenwen
    Perepelov, Andrei V.
    Weintraub, Andrej
    Liu, Bin
    Widmalm, Goeran
    Knirel, Yuriy A.
    CARBOHYDRATE RESEARCH, 2016, 427 : 44 - 47
  • [9] Structural characterization of the lipopolysaccharide O-antigen and capsular polysaccharide of Vibrio ordalii serotype O:2
    Sadovskaya, I
    Brisson, JR
    Khieu, NH
    Mutharia, LM
    Altman, E
    EUROPEAN JOURNAL OF BIOCHEMISTRY, 1998, 253 (01): : 319 - 327
  • [10] Structural characterization of the lipopolysaccharide O-polysaccharide antigen produced by Flavobacterium columnare ATCC 43622
    MacLean, LL
    Perry, MB
    Crump, EM
    Kay, WW
    EUROPEAN JOURNAL OF BIOCHEMISTRY, 2003, 270 (16): : 3440 - 3446
12345