Capillary zone electrophoresis with on-line blotting for separation and detection of 32P-postlabelled DNA adducts

被引：3
|
作者：
Schmitz, O [1 ]
Richter, E [1 ]
机构：
[1] Walther Straub Inst Pharmakol & Toxikol, D-80336 Munich, Germany
来源：
BIOMARKERS | 2000年 / 5卷 / 04期
关键词：
DNA adducts; capillary electrophoresis; P-32-postlabelling;
D O I：
10.1080/135475000413863
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A new technique for the detection of P-32-postlabelled DNA adducts separated by capillary electrophoresis was developed. By direct transfer from the capillary outlet to a positively charged moving filter paper, eluted radioactive peaks can be quantified using a phosphor imaging detector. With this method it is possible to separate DNA adducts from different carcinogens after P-32-postlabelling of the modified and unmodified nucleotides with high sensitivity approaching 1 adduct per 10(9) nucleotides.
引用
收藏
页码：314 / 321
页数：8
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