Engineering Escherichia coli for fumaric acid production from glycerol

被引：48

作者：

Li, Ning ^{[1
,2
]}

Zhang, Bo ^{[1
,2
]}

Wang, Zhiwen ^{[1
,2
]}

Tang, Ya-Jie ^{[3
]}

Chen, Tao ^{[1
,2
]}

Zhao, Xueming ^{[1
,2
]}

机构：

[1] Tianjin Univ, Key Lab Syst Bioengn, Minist Educ, Tianjin 300072, Peoples R China

[2] Tianjin Univ, Collaborat Innovat Ctr Chem Sci & Engn Tianjin, Sch Chem Engn & Technol, SynBio Res Platform, Tianjin 300072, Peoples R China

[3] Hubei Univ Technol, Key Lab Fermentat Engn, Minist Educ, Wuhan 430068, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2014年 / 174卷

基金：

中国国家自然科学基金;

关键词：

Fumaric acid; Glycerol; Escherichia coli; Fed-batch culture; Acetate accumulation; SACCHAROMYCES-CEREVISIAE; BACILLUS-SUBTILIS; CRUDE GLYCEROL; GENE; FERMENTATION; METABOLISM; PATHWAYS; DISRUPTION; EXPRESSION; CLONING;

D O I：

10.1016/j.biortech.2014.09.147

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The evolved mutant Escherichia coli E2 previously developed for succinate production from glycerol was engineered in this study for fumaric acid production under aerobic conditions. Through deletion of three fumarases, 3.65 g/L fumaric acid was produced with the yield of 0.25 mol/mol glycerol and a large amount of acetate was accumulated as the main byproduct. In order to reduce acetate production several strategies were attempted, among which increasing the flux of the anaplerotic pathways through overexpression of phosphoenolpyruvate carboxylase gene ppc or the glyoxylate shunt operon aceBA effectively reduced acetate and improved fumaric acid production. In fed-batch culture, the resulting strain EF02(pSCppc) produced 41.5 g/L fumaric acid from glycerol with 70% of the maximum theoretical yield and an overall productivity of 0.51 g/L/h. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：81 / 87

页数：7

共 33 条

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