Requirement of duplicated operons for maximal metabolism of phthalate by Rhodococcus sp strain DK17

被引：11
作者：
Choi, Ki Young
Kim, Dockyu
Chae, Jong-Chan
Zystra, Gerben J.
Kim, Eungbin [1 ]
机构：
[1] Yonsei Univ, Dept Biol, Seoul 120749, South Korea
[2] Chosun Univ, Dept Environm Engn, BK21 Team Biodydrogen Prod, Kwangju 501759, South Korea
[3] Rutgers State Univ, Sch Environm & Biol Sci, Biotechnol Ctr Agr & Environm, New Brunswick, NJ 08901 USA
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2007年 / 357卷 / 03期
基金：
美国国家科学基金会;
关键词：
Rhodococcus; phthalate; gene duplication; operon; megaplasmid;
D O I：
10.1016/j.bbrc.2007.04.009
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The operons encoding the transformation of phthalate to protocatechuate are duplicated and present on two different megaplasmids [pDK2 (330 kb) and pDK3 (750 kb)] in Rhodoeoccus sp. strain DK17. RT-PCR experiments using gene-specific primers showed that both the pDK2- and the pDK3-encoded dihydroxyphthalate decarboxylase genes are simultaneously expressed during growth on phthalate. The doubling time of the pDK2-cured mutant strain DK 176 in minimal liquid medium with 5 mM phthalate is 52.5% of that of the wild-type strain DK17. The data indicate that both copies of the phthalate operon are equally functional in DK17, and gene dosage is the main reason for slower growth of DK 176 on phthalate. (c) 2007 Elsevier Inc. All rights reserved.
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页码：766 / 771
页数：6
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