Evaluation of different subcellular fractions of Vibrio cholerae O139 in protection to challenge in experimental cholera

被引：5
作者：
Bondre, VP [1 ]
Sinha, VB [1 ]
Srivastava, BS [1 ]
机构：
[1] Cent Drug Res Inst, Div Microbiol, Lucknow 226001, Uttar Pradesh, India
来源：
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY | 1997年 / 19卷 / 04期
关键词：
Vibrio cholerae O139; subcellular fraction; immunization; protection;
D O I：
10.1016/S0928-8244(97)00099-0
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Various cellular fractions of Vibrio cholerae O139 were prepared and evaluated in the rabbit ileal loop model of experimental cholera for identification of the protective antigen(s) relevant for vaccine development. Lipopolysaccharides (LPS) and capsular polysaccharides (CPS) of O139 strains and its cell surface, membrane and cytosolic fractions were assayed for antibacterial immunity, whereas the cholera toxin was examined for antitoxic immunity. The lipopolysaccharides, membrane fraction and cholera toxin induced moderate protection, however there was a significant synergistic effect when cholera toxin was combined with membrane proteins or lipopolysaccharides. The O139 strains strongly resembled O1 strains in the profile of proteins and immunological cross reactivity, yet there was no cross protection. The results warrant further investigation of the pathogenesis of O139 strains and identify the critical somatic antigens relevant to protection. (C) 1998 Published by Elsevier Science B.V. All rights reserved.
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收藏
页码：323 / 329
页数：7
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