Hemozoin is a product of heme detoxification in the gut of the most medically important species of the family Opisthorchiidae

被引：17

作者：

Lvova, Maria ^{[1
]}

Zhukova, Mariya ^{[1
]}

Kiseleva, Elena ^{[1
]}

Mayboroda, Oleg ^{[2
,3
]}

Hensbergen, Paul ^{[2
]}

Kizilova, Elena ^{[1
]}

Ogienko, Anna ^{[1
]}

Besprozvannykh, Vladimir ^{[4
]}

Sripa, Banchob ^{[5
]}

Katokhin, Alexey ^{[1
]}

Mordvinov, Viatcheslav ^{[1
,6
]}

机构：

[1] Russian Acad Sci, Siberian Branch, Fed State Res Ctr, Inst Cytol & Genet, 10 Lavrentiev Ave, Novosibirsk 630090, Russia

[2] Leiden Univ, Med Ctr, Ctr Prote & Metabol, Zone P,Albinusdreef 2, NL-2333 ZA Leiden, Netherlands

[3] Tomsk State Univ, Dept Chem, 2 Moskovsky Trakt, Tomsk 634050, Russia

[4] Russian Acad Sci, Far Eastern Branch, Inst Biol & Soil Sci, Stoletiya St 159, Vladivostok 690022, Russia

[5] Khon Kaen Univ, Fac Med, Dept Pathol, 123 Mittraparp Highway, Khon Kaen 40002, Thailand

[6] Russian Acad Med Sci, Siberian Branch, Inst Mol Biol & Biophys, Lab Pharmacokinet & Drugs Metab, Timakov St 2-12, Novosibirsk 630117, Russia

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY | 2016年 / 46卷 / 03期

基金：

俄罗斯基础研究基金会;

关键词：

Opisthorchis felineus; Opisthorchis viverrini; Clonorchis sinensis; Hemozoin; Lipid droplets; Blood-feeding parasite; PLASMODIUM HEMOZOIN; SCHISTOSOMA-MANSONI; BLOOD; HAEMOZOIN; PARASITES; TEGUMENT; PROTEIN;

D O I：

10.1016/j.ijpara.2015.12.003

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Many species of trematodes such as Schistosoma spp., Fasciola hepatica and Echinostoma trivolvis are blood-feeding parasites. Nevertheless, there is no consensus on the feeding habits of the family Opisthorchiidae (Opisthorchis felineus, Opisthorchis viverrini and Clonorchis sinensis). Previously, histological studies of O. felineus and C. sinensis revealed some dark stained material in their gut lumen. In this study we conducted a comprehensive analysis of the gut contents of three members of the family Opisthorchiidae (O. felineus, O. viverrini and C sinensis). Using transmission electron microscopy, we demonstrated for the first known time the presence of disintegrating blood cells in the gut of O. felineus as well as electron-dense crystals in the gut of O. felineus and C sinensis. Electron energy loss spectroscopy revealed iron atoms in these crystals, and mass spectrometry of the purified pigment demonstrated the presence of heme. Fourier-transform infrared spectroscopy identified the signature peaks of the common iron-carboxylate bond characteristic in crystals isolated from O. felineus and C. sinensis. Scanning electron microscopy showed layered ovoid crystals of various sizes from 50 nm to 2 mu m. Morphological, chemical and paramagnetic properties of these crystals were similar to those of hemozoin from Schistosoma mansoni. Crystal formation occurs on the surface of lipid droplets in O. felineus and C. sinensis guts. Our results suggest that the diet of O. felineus and C. sinensis includes blood. Detoxification of the free heme produced during the digestion proceeds via formation of insoluble crystals that contain iron and heme dimers, i.e. crystals of hemozoin. Furthermore, we believe that biocrystallisation of hemozoin takes place on the surface of the lipid droplets, similar to S. mansoni. Hemozoin was not detected in the closely related species O. viverrini. (C) 2016 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.

引用

页码：147 / 156

页数：10

共 41 条

[1]

Al'perovich B.I., 1989, TREATMENT SURG COMPL, P106

[2] Synthetic Hemozoin (β-Hematin) Crystals Nucleate at the Surface of Neutral Lipid Droplets that Control Their Sizes [J].