Isolation and characterisation of polymorphic microsatellite markers for the endangered ferreous limpet Patella ferruginea (Gastropoda, Patellidae)

被引：5

作者：

Machordom, A. ^{[1
]}

Ramirez-Escobar, U. ^{[1
]}

Acevedo, I. ^{[1
]}

Garcia-Jimenez, R. ^{[1
]}

Cabezas, P. ^{[1
]}

Calvo, M. ^{[1
]}

Toledo, C. ^{[1
]}

Bloor, P. ^{[1
]}

机构：

[1] CSIC, Museo Nacl Ciencias Nat, E-28006 Madrid, Spain

来源：

CONSERVATION GENETICS | 2010年 / 11卷 / 03期

关键词：

Endangered species; Ferrous limpet; Patella ferruginea; CONSERVATION; LOCI;

D O I：

10.1007/s10592-009-9813-4

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

The limpet Patella ferruginea is one of the most endangered marine invertebrates on western Mediterranean rocky shores. We have isolated and characterised 11 polymorphic microsatellites markers to provide new tools to investigate genetic structure and gather information necessary for the proper management of this severely threatened species. The number of alleles per locus ranged from 2 to 16 (mean; Na = 8.37) with an average observed heterozygosity of 0.64 (He = 0.66). The levels of polymorphism uncovered at these loci suggest that they should be useful for population genetic studies, parentage analysis and assessment of connectivity among protected areas. None of the pairwaise comparisons among loci showed significant linkage disequilibrium after sequential Bonferroni correction. All but one locus (Pf-31IF1) conformed to HW equilibrium. Further investigation revealed that departures at Pf-31IF1 were not caused by null alleles. Results from cross-species amplifications suggest that some of these loci may also be useful for Patella tenuis (two loci) P. ulyssiponensis (one locus) and P. piperata (three loci).

引用

页码：1083 / 1086

页数：4

共 10 条

[1] Development of tetranucleotide microsatellite markers for the cushion star, Asterina gibbosa, and cross-species amplification
Acevedo, I.
Bloor, P.
Cabezas, P.
Toledo, C.
Calvo, M.
Machordom, A.
[J]. MOLECULAR ECOLOGY RESOURCES, 2009, 9 (01) : 274 - 277
[2] Highly polymorphic tetranucleotide microsatellite loci for the eastern Canary Island lizard, Gallotia atlantica
Bloor, P.
De laguna, I. H. -B.
Kemp, S. J.
[J]. MOLECULAR ECOLOGY NOTES, 2006, 6 (03): : 737 - 739
[3] Population genetics of the endangered limpet Patella ferruginea (Gastropoda: Patellidae):: taxonomic, conservation and evolutionary considerations
Espinosa, F
Ozawa, T
[J]. JOURNAL OF ZOOLOGICAL SYSTEMATICS AND EVOLUTIONARY RESEARCH, 2006, 44 (01) : 8 - 16
[4] Aspects of reproduction and their implications for the conservation of the endangered limpet, Patella ferruginea
Espinosa, Free
Guerra-Garcia, Jose M.
Faz, Darren
Garcia-Gomez, J. Carlos
[J]. INVERTEBRATE REPRODUCTION & DEVELOPMENT, 2006, 49 (1-2) : 85 - 92
[5] Phylogeny and historical biogeography of limpets of the order Patellogastropoda based on mitochondrial DNA sequences
Nakano, T
Ozawa, T
[J]. JOURNAL OF MOLLUSCAN STUDIES, 2004, 70 : 31 - 41
[6] GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research
Peakall, R
Smouse, PE
[J]. MOLECULAR ECOLOGY NOTES, 2006, 6 (01): : 288 - 295
[7] Development of microsatellite loci for the black-footed limpet, Patella depressa, and cross-amplification in two other Patella species
Perez, M.
Branco, M.
Llavona, A.
Ribeiro, P. A.
Santos, A. M.
Hawkins, S. J.
Davila, J. A.
Presa, P.
Alexandrino, P.
[J]. CONSERVATION GENETICS, 2007, 8 (03) : 739 - 742
[8] ANALYZING TABLES OF STATISTICAL TESTS
RICE, WR
[J]. EVOLUTION, 1989, 43 (01) : 223 - 225
[9] Rozen S, 2000, Methods Mol Biol, V132, P365
[10] Templado J., 2004, Guia de los invertebrados peces marinos espanoles protegidos por la legislacion nacional internacional

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