Determination of Phenols in Fructus Lycii by Capillary Electrophoresis with Electrochemical Detection

A high-performance capillary electrophoresis with electrochemical detection (CE-ED) method has been developed for the determination of bioactive phenols in Fructus Lycii samples. Under the optimum conditions, the seven analytes could be well separated within 24 min in a 80 mM borax running buffer. Good linear relationship was established between peak current and concentration of analytes over three orders of magnitude with detection limits (S/N = 3) ranging from 3.5 x 10(-8) to 6.0 x 10(-8) g/mL for all analytes. The proposed method has been successfully applied for the study on the efficiency of electrochemically active ingredients in polar extracts of Fructus Lycii after a relatively simple pretreatment.