IL-8/CXCR2 Signalling Promotes Cell Proliferation in Oesophageal Squamous Cell Carcinoma and Correlates With Poor Prognosis

被引：8
作者：
Inoue, Masazumi [1 ]
Takeuchi, Hiroya [2 ]
Matsuda, Sachiko [1 ]
Nishi, Tomohiko [1 ]
Fukuda, Kazumasa [1 ]
Nakamura, Rieko [1 ]
Takahashi, Tsunehiro [1 ]
Wada, Norihito [1 ]
Kawakubo, Hirofumi [1 ]
Kitagawa, Yuko [1 ]
机构：
[1] Keio Univ, Sch Med, Dept Surg, Tokyo, Japan
[2] Hamamatsu Univ, Sch Med, Dept Surg, Shizuoka, Japan
来源：
ANTICANCER RESEARCH | 2021年 / 41卷 / 02期
关键词：
Oesophageal squamous cell carcinoma; IL-8; CXCR2; chemokine; cell proliferation; WNT ANTAGONIST SFRP1; PHASE-III TRIAL; CXCR2; EXPRESSION; POSTOPERATIVE COMPLICATIONS; GASTROESOPHAGEAL JUNCTION; PREOPERATIVE CHEMOTHERAPY; EPIGENETIC INACTIVATION; CANCER; GROWTH; INTERLEUKIN-8;
D O I：
10.21873/anticanres.14830
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Background/Aim: The inflammatory cytokine IL-8 and its receptor CXCR2 are key signalling pathway molecules in cancer development. We hypothesized that IL-8/CXCR2 signalling promotes tumour progression in oesophageal squamous cell carcinoma (ESCC) patients. Materials and Methods: We examined the relationship between IL-8/CXCR2 expression and clinicopathological factors by inununohistochemistry in samples from 63 patients with resectable ESCC. The effects of IL-8/CXCR2 signalling on cell proliferation and gene expression were examined in vitro and in vivo using ESCC cell lines. Results: Increased IL-8/CXCR2 signalling was associated with shorter overall survival (p<0.05) and recurrence-free survival (p<0.05) in ESCC patients. Multivariate analysis identified IL-8/CXCR2 expression as a prognostic factor for surgically treated ESCC (p<0.05). In vitro, IL-8 exposure or over-expression significantly enhanced ESCC cell proliferation. SB225002, a CXCR2-specific antagonist, and IL-8 siRNA significantly suppressed cell proliferation. Conclusion: IL-8/CXCR2 expression is an independent prognostic factor for surgically treated ESCC, and IL-8/CXCR2 signalling contributes to ESCC cell proliferation.
引用
收藏
页码：783 / 794
页数：12
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