Purification and biological evaluation of the metabolites produced by Streptomyces sp TK-VL_333

被引:42
作者
Kavitha, Alapati [1 ]
Prabhakar, Peddikotla [2 ]
Vijayalakshmi, Muvva [1 ]
Venkateswarlu, Yenamandra [2 ]
机构
[1] Acharya Nagarjuna Univ, Dept Bot & Microbiol, Guntur 522510, India
[2] Indian Inst Chem Technol, Organ Chem Div 1, Hyderabad 500007, Andhra Pradesh, India
关键词
Actinobacteria; Streptomyces sp TK-VL_333; Bioactive metabolites; 1H-indole-3-carboxylic acid; 2,3-Dihydroxy-5-(hydroxymethyl) benzaldehyde; Acetic acid-2-hydroxy-6-(3-oxo-butyl)-phenyl ester; DRUG DISCOVERY; NUMERICAL CLASSIFICATION; BIOSYNTHETIC CAPACITIES; AUREOFACIENS CMUAC130; SECONDARY METABOLITES; BIOACTIVE METABOLITES; ANTIFUNGAL ACTIVITY; SELECTIVE ISOLATION; BIOCONTROL AGENT; SOIL;
D O I
10.1016/j.resmic.2010.03.011
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
An Actinobacterium strain isolated from laterite soils of the Guntur region was identified as Streptomyces sp. TK-VL_333 by 16S rRNA analysis. Cultural, morphological and physiological characteristics of the strain were recorded. The secondary metabolites produced by the strain cultured on galactose-tyrosine broth were extracted and concentrated followed by defatting of the crude extract with cyclohexane to afford polar and non-polar residues. Purification of the two residues by column chromatography led to isolation of five polar and one non-polar fraction. Bioactivity- guided fractions were rechromatographed on a silica gel column to obtain four compounds, namely 1H-indole-3-carboxylic acid, 2,3-dihydroxy-5-(hydroxymethyl) benzaldehyde, 4-(4-hydroxyphenoxy) butan-2-one and acetic acid-2-hydroxy-6-(3-oxo-butyl)-phenyl ester from three active polar fractions and 8-methyl decanoic acid from one non-polar fraction. The structure of the compounds was elucidated on the basis of FT-IR, mass and NMR spectroscopy. The antimicrobial activity of the bioactive compounds produced by the strain was tested against the bacteria and fungi and expressed in terms of minimum inhibitory concentration. Antifungal activity of indole-3-carboxylic acid was further evaluated under in vitro and in vivo conditions. This is the first report of 2,3-dihydroxy-5-(hydroxymethyl) benzaldehyde, 4-(4-hydroxyphenoxy) butan-2-one, acetic acid-2-hydroxy-6-(3-oxo-butyl)-phenyl ester and 8-methyl decanoic acid from the genus Streptomyces. (C) 2010 Elsevier Masson SAS. All rights reserved.
引用
收藏
页码:335 / 345
页数:11
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