Novel fluorescent protein from Hydnophora rigida possess cyano emission

被引：1

作者：

Bokhari, H. ^{[2
]}

Smith, C. ^{[1
]}

Veerendra, K. ^{[3
]}

Sivaraman, J. ^{[3
]}

Sikaroodi, M. ^{[2
]}

Gillevet, P. ^{[2
]}

机构：

[1] USA, Engineer Res & Dev Ctr, Alexandria, VA USA

[2] George Mason Univ, Dept Environm Sci & Policy, Microbiome Anal Ctr, Manassas, VA USA

[3] Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 396卷 / 03期

关键词：

Functional cloning; Cyano fluorescent protein; Coral; Hydnophora rigida; Prokaryotic expression; AEQUOREA; ANTHOZOA; FAMILY;

D O I：

10.1016/j.bbrc.2010.04.136

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Currently, a broad diversity of fluorescent proteins among marine organisms range from cyano-red emissions. Fluorescent proteins differ in their DNA sequences from green fluorescent protein (GFP). We identified cDNA encoding the gene of a new protein from the reef coral Hydnophora rigida of the Merulinidae family. Both the spectral properties and putative primary sequence of the protein has been determined. The cloned cDNA encode peptide we call HriCFP is comprised of 134 amino acids. It has characteristics of a cyano fluorescent protein (HriCFP) and its sequence is markedly different from known GFP from the hydroid jellyfish Aequorea victoria. HriCFP was cloned, expressed, purified and exist as monomer. The peptide mass finger print on the purified protein confirmed identity of HriCFP. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：631 / 636

页数：6

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