Highly selective recognition and fluorescent detection of JEV via virus-imprinted magnetic silicon microspheres

被引：40
作者：
He, Kui [1 ]
Chen, Chunyan [1 ]
Liang, Caishuang [1 ]
Liu, Chan [1 ]
Yang, Bin [1 ]
Chen, Xiaoming [1 ]
Cai, Changqun [1 ]
机构：
[1] Xiangtan Univ, Coll Chem, Minist Educ, Key Lab Environm Friendly Chem & Applicat, Xiangtan 411105, Hunan, Peoples R China
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2016年 / 233卷
基金：
中国国家自然科学基金;
关键词：
Japanese encephalitis virus; Surface imprinting; Fluorescent detection; Virus-imprinted magnetic silicon microspheres; INFLUENZA-VIRUS; POLYMERS; PROTEIN; STRATEGY; BINDING;
D O I：
10.1016/j.snb.2016.04.127
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A novel virus-recognition fluorescent sensor based on virus-imprinted polymers (VIP) for highly selective and highly sensitive detection of Japanese encephalitis virus (JEV) was presented. The virus-imprinted method went beyond simple shape imprinting through surface imprinting with magnetic silicon micro spheres acting as carrier materials and tetraethyl orthosilicate (TEOS) functioning as building blocks. The resulting VIP film could selectively capture target JEV via recognition cavities, and a high imprinting factor of 2.98 was achieved. Furthermore, fast magnetic separation and highly sensitive fluorescent detection in water were offered for JEV, showing a good linearity within 2.5-45 nM (R-2 = 0.9948). Finally, the specificity study confirmed that the virus-imprinted magnetic silicon microspheres demonstrated excellent selectivity for JEV in several different types of virus. (C) 2016 Elsevier B.V. All rights reserved.
引用
收藏
页码：607 / 614
页数：8
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