Passive Immunization with LINGO-1 Polyclonal Antiserum Afforded Neuroprotection and Promoted Functional Recovery in a Rat Model of Spinal Cord Injury

被引：21

作者：

Lv, Jun ^{[1
]}

Xu, Ru-xiang ^{[1
]}

Jiang, Xiao-dan ^{[1
]}

Lu, Xin ^{[2
]}

Ke, Yi-quan ^{[1
]}

Cai, Ying-qian ^{[1
]}

Du, Mou-xuan ^{[1
]}

Hu, Changchen ^{[1
]}

Zou, Yu-xi ^{[1
]}

Qin, Ling-sha ^{[1
]}

Zeng, Yan-jun ^{[3
]}

机构：

[1] So Med Univ, Zhujiang Hosp, Neurosurg Inst Guangdong, Dept Neurosurg, Guangzhou 510282, Guangdong, Peoples R China

[2] So Med Univ, Zhujiang Hosp, Dept Endocrinol, Guangzhou 510282, Guangdong, Peoples R China

[3] Beijing Univ Technol, Biomech & Med Informat Inst, Beijing, Peoples R China

来源：

NEUROIMMUNOMODULATION | 2010年 / 17卷 / 04期

关键词：

LINGO-1; Passive immunization; Spinal cord injury; RhoA; Neuron apoptosis; Functional recovery; CENTRAL-NERVOUS-SYSTEM; AXONAL REGENERATION; INHIBITOR; RECEPTOR; RHO;

D O I：

10.1159/000290043

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

LINGO-1 (leucine-rich repeat and Ig domain-containing, Nogo receptor-interacting protein) is an important component of the NgR receptor complex involved in RhoA activation and axon regeneration. The authors report on passive immunization with LINGO-1 polyclonal antiserum, a therapeutic approach to overcome NgR-mediated growth inhibition after spinal cord injury (SCI). The intrathecally administered high-titer rabbit-derived antiserum can be detected around the injury site within a wide time window; it blocks LINGO-1 in vivo with high molecular specificity. In this animal model, passive immunization with LINGO-1 antiserum significantly decreased RhoA activation and increased neuronal survival. Adult rats immunized in this manner show recovery of certain hindlimb motor functions after dorsal hemisection of the spinal cord. Thus, passive immunotherapy with LINGO-1 polyclonal antiserum may represent a promising repair strategy following acute SCI. Copyright (C) 2010 S. Karger AG, Basel

引用

页码：270 / 278

页数：9

共 13 条

[1] Central nervous system and non-central nervous system antigen vaccines exacerbate neuropathology caused by nerve injury
Ankeny, Daniel P.
Popovich, Phillip G.
[J]. EUROPEAN JOURNAL OF NEUROSCIENCE, 2007, 25 (07) : 2053 - 2064
[2] Axonal growth therapeutics: regeneration or sprouting or plasticity?
Cafferty, William B. J.
Mcgee, Aaron W.
Strittmatter, Stephen M.
[J]. TRENDS IN NEUROSCIENCES, 2008, 31 (05) : 215 - 220
[3] Rho activation patterns after spinal cord injury and the role of activated Rho in apoptosis in the central nervous system
Dubreuil, CI
Winton, MJ
McKerracher, L
[J]. JOURNAL OF CELL BIOLOGY, 2003, 162 (02) : 233 - 243
[4] A therapeutic vaccine approach to stimulate axon regeneration in the adult mammalian spinal cord
Huang, DW
McKerracher, L
Braun, PE
David, S
[J]. NEURON, 1999, 24 (03) : 639 - 647
[5] LINGO-1 antagonist promotes functional recovery and axonal sprouting after spinal cord injury
Ji, Benxiu
Li, Mingwei
Wu, Wu-Tian
Yick, Leung-Wah
Lee, Xinhua
Shao, Zhaohui
Wang, Joy
So, Kwok-Fai
McCoy, John M.
Pepinsky, R. Blake
Mi, Sha
Relton, Jane K.
[J]. MOLECULAR AND CELLULAR NEUROSCIENCE, 2006, 33 (03) : 311 - 320
[6] Kubo T, 2007, CURR PHARM DESIGN, V13, P2493
[7] Monoclonal antibodies come of age
Ledford, Heidi
[J]. NATURE, 2008, 455 (7212) : 437 - 437
[8] LINGO-1 is a component of the Nogo-66 receptor/p75 signaling complex
Mi, S
Lee, X
Shao, ZH
Thill, G
Ji, BX
Relton, J
Levesque, M
Allaire, N
Perrin, S
Sands, B
Crowell, T
Cate, RL
McCoy, JM
Pepinsky, RB
[J]. NATURE NEUROSCIENCE, 2004, 7 (03) : 221 - 228
[9] LINGO-1 and its role in CNS repair
Mi, Sha
Sandrock, Alfred
Miller, Robert H.
[J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, 2008, 40 (10) : 1971 - 1978
[10] A NOVEL ROLE FOR MYELIN-ASSOCIATED GLYCOPROTEIN AS AN INHIBITOR OF AXONAL REGENERATION
MUKHOPADHYAY, G
DOHERTY, P
WALSH, FS
CROCKER, PR
FILBIN, MT
[J]. NEURON, 1994, 13 (03) : 757 - 767

← 1 2 →