Immunogenicity of a subunit vaccine against Bacillus anthracis

被引：61

作者：

Chichester, Jessica A. ^{[1
]}

Musiychuk, Konstantin ^{[1
]}

de la Rosa, Patricia ^{[1
]}

Horsey, April ^{[1
]}

Stevenson, Natalie ^{[1
]}

Ugulava, Natalia ^{[1
]}

Rabindran, Shailaja ^{[1
]}

Palmer, Gene A. ^{[1
]}

Mett, Vadim ^{[1
]}

Yusibov, Vidadi ^{[1
]}

机构：

[1] Fraunhofer USA Ctr Mol Biotechnol, Newark, DE 19711 USA

来源：

VACCINE | 2007年 / 25卷 / 16期

关键词：

anthrax; subunit vaccine; plant expression;

D O I：

10.1016/j.vaccine.2007.01.068

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The current approved vaccine against anthrax is based on protective antigen (PA) of Bacillus anthracis, requires six injections over an 18-month period and has a known history of side effects. Therefore, there is significant effort towards developing an improved vaccine against B. anthracis. Here we separately engineered and expressed domain 4 of PA (PAD4) and domain I of lethal factor (LFD1) as fusions to lichenase (LicKM), a thermostable enzyme from Clostridium thermocellum, and transiently expressed these fusions in Nicotiana benthamiana. Plant-produced antigens were combined and immunogenicity was evaluated in mice. All animals that received the experimental vaccine developed high antibody titers that were predominantly IgGI and were able to neutralize the effects of LeTx in vitro. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：3111 / 3114

页数：4

共 13 条

[1] Anthrax toxin: a tripartite lethal combination [J].

Ascenzi, P ;

Visca, P ;

Ippolito, G ;

Spallarossa, A ;

Bolognesi, M ;

Montecucco, C .

FEBS LETTERS, 2002, 531 (03) :384-388

[2] Functional analysis of Bacillus anthracis protective antigen by using neutralizing monoclonal antibodies [J].

Brossier, F ;

Lévy, M ;

Landier, A ;

Lafaye, P ;

Mock, M .

INFECTION AND IMMUNITY, 2004, 72 (11) :6313-6317

[3] A recombinant carboxy-terminal domain of the protective antigen of Bacillus anthracis protects mice against anthrax infection [J].

Flick-Smith, HC ;

Walker, NJ ;

Gibson, P ;

Bullifent, H ;

Hayward, S ;

Miller, J ;

Titball, RW ;

Williamson, ED .

INFECTION AND IMMUNITY, 2002, 70 (03) :1653-1656

[4] Protection against anthrax toxin by vaccination with a DNA plasmid encoding anthrax protective antigen [J].

Gu, ML ;

Leppla, SH ;

Klinman, DM .

VACCINE, 1999, 17 (04) :340-344

[5] Human-derived, plant-produced monoclonal antibody for the treatment of anthrax [J].

Hull, AK ;

Criscuolo, CJ ;

Mett, V ;

Groen, H ;

Steeman, W ;

Westra, H ;

Chapman, G ;

Legutki, B ;

Baillie, L ;

Yusibov, V .

VACCINE, 2005, 23 (17-18) :2082-2086

[6] RECENT ADVANCES IN THE DEVELOPMENT OF AN IMPROVED, HUMAN ANTHRAX VACCINE [J].

IVINS, BE ;

WELKOS, SL .

EUROPEAN JOURNAL OF EPIDEMIOLOGY, 1988, 4 (01) :12-19

[7] Plant-based vaccine: Mice immunized with chloroplast-derived anthrax protective antigen survive anthrax lethal toxin challenge\ [J].

Koya, V ;

Moayeri, M ;

Leppla, SH ;

Daniell, H .

INFECTION AND IMMUNITY, 2005, 73 (12) :8266-8274

[8] An anthrax lethal factor-neutralizing monoclonal antibody protects rats before and after challenge with anthrax toxin [J].

Lim, NK ;

Kim, JH ;

Oh, MS ;

Lee, S ;

Kim, SY ;

Kim, KS ;

Kang, HJ ;

Hong, HJ ;

Inn, KS .

INFECTION AND IMMUNITY, 2005, 73 (10) :6547-6551

[9] A launch vector for the production of vaccine antigens in plants [J].

Musiychuk, Konstantin ;

Stephenson, Natalie ;

Bi, Hong ;

Farrance, Christine E. ;

Orozovic, Goran ;

Brodelius, Maria ;

Brodelius, Peter ;

Horsey, April ;

Ugulava, Natalia ;

Shamloul, Abdel-Moneim ;

Mett, Vadim ;

Rabindran, Shailaja ;

Streatfield, Stephen J. ;

Yusibov, Vidadi .

INFLUENZA AND OTHER RESPIRATORY VIRUSES, 2007, 1 (01) :19-25

[10] PROTECTIVE IMMUNITY INDUCED BY BACILLUS-ANTHRACIS TOXIN-DEFICIENT STRAINS [J].

PEZARD, C ;

WEBER, M ;

SIRARD, JC ;

BERCHE, P ;

MOCK, M .

INFECTION AND IMMUNITY, 1995, 63 (04) :1369-1372

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