A new inhibitor of the transcription-termination factor Rho

被引：2
|
作者：
Carrano, L
Alifano, P
Corti, E
Bucci, C
Donadio, S
机构：
[1] Biosearch Italia, I-21040 Gerenzano, VA, Italy
[2] Univ Lecce, Dipartimento Sci & Tecnol Biol & Ambientali, I-73100 Lecce, Italy
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 302卷 / 02期
关键词：
antibiotic bicyclomycin; RNA; screening;
D O I：
10.1016/S0006-291X(03)00131-1
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In this study we describe BI-K0058, a new inhibitor of the transcription-termination factor Rho belonging to a different chemical class from bicyclomycin, the only known antibiotic acting on Rho. BI-K0058 inhibits the poly(C)-dependent ATPase activity of Rho with an IC50 of 25 muM as well as in vitro transcription-termination of two natural substrates, the Salmonella enterica hisG cistron and the f1 phage intergenic region. BI-K0058 does not affect photolabeling of Rho by ATP. The results of gel mobility shift experiments with a natural RNA substrate demonstrate that BI-K0058 inhibits the formation of the ATP-independent high affinity Rho-RNA complex. (C) 2003 Elsevier Science (USA). All rights reserved.
引用
收藏
页码：219 / 225
页数：7
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