Interferon gamma and lipopolysaccharide upregulate TNF-related apoptosis-inducing ligand expression in murine microglia

被引：20

作者：

Genc, S ^{[1
]}

Kizyldag, S

Genc, K

Ates, H

Atabey, N

机构：

[1] Dokuz Eylul Univ, Sch Med, Dept Med Biol & Med Genet, TR-35340 Izmir, Turkey

[2] Dokuz Eylul Univ, Sch Med, Dept Hematol & Oncol, TR-35340 Izmir, Turkey

[3] Dokuz Eylul Univ, Sch Med, Dept Physiol, TR-35340 Izmir, Turkey

来源：

IMMUNOLOGY LETTERS | 2003年 / 85卷 / 03期

关键词：

TRAIL; microglia; N9 cell line; murine; neurodegeneration;

D O I：

10.1016/S0165-2478(02)00245-6

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In this study, it is reported that neonatal murine microglia and N9 murine microglial cell line express tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL). TRAIL protein and mRNA expression in murine microglia greatly upregulate upon stimulation with interferon gamma (IFNgamma) or lipopolysaccharide (LPS) as revealed by immunoprecipitation-immunoblotting, reverse transcriptase-polymerase chain reaction (RT-PCR) and flow cytometry techniques. IFNgamma and LPS act synergistically to induce TRAIL expression on both translational and transcriptional levels. The upregulated microglial TRAIL in inflammatory conditions may involve in the cytotoxic effect of these cells and play a role in neurodegenerative processes. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：271 / 274

页数：4

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