Bound energy of water in hard dental tissues

Enamel and dentin are composed, respectively, of 3 wt% and 10 wt% of water, which exhibits different features in the tissues: loosely and tightly bound water. The objective of this study is to clarify by infrared spectroscopy, the different features of the water in heated (100-1000degreesC) hard dental tissues (enamel and dentin). The water band between 3800cm(-1) and 2500cm(-1) was analyzed by infrared spectroscopy. The area dependence of the water band with temperature was compared with the Arrhenius equation in two regions (100-400degreesC and 700-1000degreesC). The activation energy was determined for these two regions, and similar values were observed for both tissues. For enamel we obtain -4.1 +/- 0.2kJ/mol at 100-400degreesC and -63 +/- 9 kJ/mol at 700-1000degreesC; for dentin -4.1 +/- 0.2 kJ/mol at 100-400degreesC and - 60 +/- I I kJ/mol at 700-1000degreesC. The water loss changes the color of the tissues, hydroxyapatite crystallographic parameters, and produce ESR signals. These changes were discussed and compared with the results observed in this work and after laser irradiation. We conclude that these two activation energies could be assigned to the adsorbed (loosely bound) and trapped (tightly bound) water.